基于细菌多样性对蔬菜发酵制品分析评价Analysis and Evaluation of Vegetable Fermented Products Based on Bacterial Diversity
王倩倩,邱泳明,迟雪梅,贾倩雯,任楠楠,迟乃玉,张庆芳
摘要(Abstract):
以草菇和白菜为基本原料发酵制得4种发酵制品,采用高通量测序技术,对其细菌多样性及功能进行分析。结果显示:以白菜为原料添加酸奶的发酵制品物种丰度最大;草菇发酵制品的细菌多样性大于白菜发酵制品。发酵制品中的细菌主要为乳酸菌;以草菇为原料的优势菌是Lactobacillus plantarum,其中添加酸奶发酵的丰度为67.63%,自然发酵的丰度为69.46%,其次是Leuconostoc pseudomesenteroides,其中添加酸奶发酵的丰度为29.51%,自然发酵的丰度为30.10%;以白菜为原料的优势菌主要是Lactobacillus plantarum,其中添加酸奶发酵的丰度为88.97%,自然发酵的丰度为90.65%。功能预测分析,4种发酵制品都有安全性和营养性的特点。文章还进一步讨论分析了蔬菜发酵制品中乳酸菌的功能及白菜发酵制品Lactobacillus plantarum为优势菌群的原因。
关键词(KeyWords): 草菇和白菜;发酵制品;细菌多样性;功能预测
基金项目(Foundation): 辽宁省自然科学基金(2014020134);; 国家重点研发计划海洋关键技术与装备领域课题(2018YFC0311100)资助项目
作者(Author): 王倩倩,邱泳明,迟雪梅,贾倩雯,任楠楠,迟乃玉,张庆芳
参考文献(References):
- [1]王冀宁,熊哲超,王雯熠.食用酱油铁含量的食品安全风险分析及对策影响[J].中国调味品,2021,46(1):166-169,176.
- [2]ZHANG Weiwei,TIAN Guoting,FENG Shanshan,et al.Boletus edulis nitrite reductase reduces nitrite content of pickles and mitigates intoxication in nitrite-intoxicated mice[J].Sci Rep,2015,5:14907.
- [3]PARK K Y,JEONG J K,LEE Y E,et al.Health benefits of kimchi(Korean fermented vegetables)as a probiotic food[J].J Med Food,2014,17:6-20.
- [4]PATRA J K,GITISHREE D,PARAMITHIOTIS S,et al.Kimchi and other widely consumed traditional fermented foods of Korea:a review[J].Front Microbiol,2016,7:1493.
- [5]毛海立,代文,杨艳.发酵食品的风味物质及其检测方法研究进展[J].广州化工,2017,45(18):10-13,27.
- [6]迟雪梅,张庆芳,迟乃玉.发酵蔬菜安全性的研究进展[J].中国酿造,2018,37(8):5-8.
- [7]MATALLANA G M C,MARTíNEZ-TOMéM J,TORIJA I M E.Nitrate and nitrite content in organically cultivated vegetables[J].Food Addit Contam:Part B Surveill,2010,3(1):19-29.
- [8]CASTELINO M,EYRE S,MOAT J,et al.Optimisation of methods for bacterial skin microbiome investigation:primer selection and comparison of the 454versus MiSeq platform[J].BMC Microbiol,2017,17:23.
- [9]EDGAR R C.Search and clustering orders of magnitude faster than BLAST[J].Bioinformatics,2010,26:2460-2461.
- [10]CHEN H B,PAUL C B.Venn diagram:apackage for the generation of highly-customizable Venn and Euler diagrams in R[J].BMC Bioinformatics,2011,12(1):35.
- [11]PARKS D H,TYSON G W,HUGENHOLTZ P,et al.STAMP:statistical analysis of taxonomic and functional profiles[J].Bioinformatics,2014,30:3123-3124.
- [12]谭海刚,李静,申美容.液态发酵米醋稳定性研究[J].中国调味品,2020,45(2):32-35.
- [13]BARRETO F M,COLADO S,ANDRéA N,et al.Beneficial effects of Lactobacillus plantarum on glycemia and homocysteine levels in postmenopausal women with metabolic syndrome[J].Nutrition,2014,30:939-942.
- [14]SHARAFEDTINOV K K,PLOTNIKOVA O A,ALEXEEVAR I,et al.Hypocaloric diet supplemented with probiotic cheese improves body mass index and blood pressure indices of obese hypertensive patients-a randomized double-blind placebocontrolled pilot study[J].Nutr J,2013,12:138.
- [15]武万强,王琳琳,赵建新,等.植物乳杆菌生理特性及益生功能研究进展[J].食品与发酵工业,2019,45(1):1-13.
- [16]迟雪梅,于爽,姜南,等.包心白菜发酵工艺筛选及营养价值评定[J].中国调味品,2017,42(12):44-49.
- [17]朱广素,王刚,王园园,等.植物乳杆菌通过调节肠道短链脂肪酸水平缓解代谢综合征[J].食品科学,2019,40(13):102-109.
- [18]贾晶晶,易洋,韩睿媛,等.高通量测序技术分析腌渍白菜液中原核微生物群落结构[J].中国调味品,2020,45(12):87-90.
- [19]TSAI Y T,CHENG P C,PAN T M.The immunomodulatory effects of lactic acid bacteria for improving immune functions and benefits[J].Applied Microbiology and Biotechnology,2012,96(4):853-862.
- [20]SHARMA M,DEVI M.Probiotics:a comprehensive approach toward health foods[J].Critical Reviews in Food Science and Nutrition,2014,54(4):537-552.
- [21]BRKICD,BOSNIR J,BEVARDI M,et al.Nitrate in leafy green vegetables and estimated intake[J].Afr J Tradit Complement Altern Med,2017,14:31-41.
- [22]DING Z,JOHANNINGSMEIER S D,PRICE R,et al.Evaluation of nitrate and nitrite contents in pickled fruit and vegetable products[J].Food Control,2018,90:304-311.
- [23]王一茜,荣金诚,王晓辉,等.常见乳酸菌降解亚硝酸盐机理探讨[J].食品与发酵工业,2019,45(8):50-56.
- [24]高吉祥,燕平梅.酱菜发酵中微生物的研究[J].中国调味品,2020,45(12):189-193.
- [25]燕平梅,王炫月,赵文婧.泡菜中亚硝酸盐形成、还原相关微生物的研究[J].中国调味品,2020,45(9):77-80,94.
- [26]迟雪梅,张庆芳.食品中常见乳酸菌高效降解NO2-发酵性能评价[J].食品与发酵工业,2017,43(6):78-84.
- [27]张庆芳,李美玉,王晓辉,等.植物乳杆菌亚硝酸盐还原酶基因在大肠杆菌中重组表达、纯化及酶学性质分析[J].食品与发酵工业,2019,45(12):28-34.
- [28]董欣旖,赵英侠.食品防腐剂在食品中应用现状分析[J].中国食品添加剂,2020,31(11):139-143.
- [29]蒙月月,陆婧婧,占萌,等.植物乳杆菌KLDS 1.0318产酸、耐酸、耐胆盐能力及其免疫特性研究[J].食品工业科技,2018,39(15):70-76.
- [30]韩宏娇,丛敏,李欣蔚,等.自然发酵酸菜化学成分含量和微生物数量的动态变化及其相关性分析[J].食品工业科技,2019,40(2):148-153.