阳凤娇,李珍艳,吴雪琪,张良,曾沛斌

• 1:四川大学华西公共卫生学院/华西第四医院
• 2:西华大学食品与生物工程学院

摘要(Abstract)：

建立了QuEChERS-高效液相色谱法测定郫县豆瓣酱中黄曲霉毒素B_1、黄曲霉毒素B_2、黄曲霉毒素G_1、黄曲霉毒素G_2和赭曲霉毒素A的快速检测方法。样品经乙腈-水(84∶16)和乙腈-水-冰乙酸(84∶15∶1)提取，分别得到黄曲霉毒素和赭曲霉毒素提取液，提取液加入QuEChERS盐包振摇离心，取上清液加入吸附剂净化处理，净化液分别经衍生或复溶后，进高效液相色谱仪分析，豆瓣酱基质匹配的真菌毒素标准曲线定量。5种真菌毒素在质量浓度范围内均呈良好线性关系，决定系数R~2>0.99,3个水平的加标实验回收率在74.26%～112.92%,相对标准偏差(RSD)为3.3%～7.2%,检出限为0.01～0.16μg/kg,定量限为0.03～0.53μg/kg。QuEChERS-HPLC具有简单、高效、经济和准确度高等优点，适用于郫县豆瓣酱中真菌毒素的快速定量分析。

关键词(KeyWords)： 豆瓣酱;真菌毒素;黄曲霉毒素;赭曲霉毒素;QuEChERS-HPLC

Abstract:

Keywords:

基金项目(Foundation): 四川省科技计划项目(2021ZHFP0162)

作者(Author): 阳凤娇,李珍艳,吴雪琪,张良,曾沛斌

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