油茶籽功能性成分的提取、鉴定及其活性评价Extraction, Identification and Activity Evaluation of Functional Components of Camellia oleifera Seeds
文卓,黄永春,唐湘毅
摘要(Abstract):
油茶籽广泛种植于我国南方地区,是食用植物油的重要原料。为了探明其有效成分,文章首先通过气相色谱-质谱法对其挥发性成分进行分析。其中,顺-十八碳烯酸、n-十六酸、7-丁基苯并[a]蒽等成分的含量较高,并发现生育酚、角鲨烯等活性成分。随后采用超声强化溶剂提取法对油茶籽中的功能性因子进行提取,并探究了超声温度、pH、乙醇浓度、料液比对活性成分提取率的影响。4个因素对酚类物质提取率的影响次序为pH>超声温度>乙醇浓度>料液比,且超声温度和乙醇浓度的交互作用最强。通过响应面优化后,得到总酚的最佳提取工艺条件为超声温度45℃、乙醇浓度40%、料液比1∶10、pH 4。抗氧化活性实验结果表明,其半抑制浓度IC_(50)为39.72 mg/mL,其在抗炎、抗肿瘤领域的功效值得进一步研究。
关键词(KeyWords): 油茶籽;索氏提取;超声强化;挥发性成分;气相色谱-质谱法(GC-MS)
基金项目(Foundation): 中国博士后科学基金项目(2023MD734187);; 广西科技基地和人才专项科学基金项目(2021AC19460)
作者(Author): 文卓,黄永春,唐湘毅
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