胡雪桃,朱瑶迪,邹小波,申婷婷,陈武,石吉勇

• 1:江苏大学食品与生物工程学院

摘要(Abstract)：

以镇江香醋固态发酵中醋醅为研究对象,研究种子醅的乙醇脱氢酶(ADH)活性,以期获得其最优活性范围,并利用近红外光谱技术结合联合区间偏最小二乘法(si-PLS)快速预测ADH活性值。研究通过测定50个不同车间不同楼层的发酵池种子醅ADH的活性,与对应接种池的总酸含量进行相关性分析,并利用近红外光谱技术对其活性值进行快速预测以及光谱分析。结果表明:对于固态发酵的整个过程,ADH活性控制在600~700U能保持食醋的良好风味。若活性低于300或高于800,总酸含量很低,易使醋醅总酸含量不达标。在一定范围内,ADH活性和总酸含量呈正相关性,相关系数R达到0.936。利用近红外光谱不同谱峰,分析醋醅中不同阶段的代谢物质,并结合si-PLS建立ADH快速预测模型,其预测集均方根误差(RMSEP)为122,相关系数R为0.8637。最终期望近红外光谱技术能够预测发酵过程,改善发酵质量。

关键词(KeyWords)： 镇江香醋;固态发酵;种子醅;乙醇脱氢酶活性;近红外光谱技术

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑项目(2015BAD19B03);; 高新技术发展计划国家863项目(2011AA100807);; 全国优秀博士基金资助项目(200968);; 国家自然科学基金(BK20130505);; 新世纪优秀人才项目(NCET-11-00986);; 杰出青年基金(BK20130010);; 江苏省研究生创新基金(KYLX-1070)

作者(Author): 胡雪桃,朱瑶迪,邹小波,申婷婷,陈武,石吉勇

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