李婧御,李元鑫,刘冉,孙永旺,郭尚敬,穆洪静,赵庆奎

• 1:聊城大学农学与农业工程学院
• 2:山东国宏生物科技有限公司

摘要(Abstract)：

利用植物多酚原花青素(oligomeric proanthocyanidins, OPC)与大豆分离蛋白(soybean protein isolate, SPI)的相互作用，采用葡萄糖酸内酯诱导结合超声波处理制备OPC-SPI凝胶，旨在改善SPI凝胶性能。研究不同浓度OPC(0.1%、0.3%、0.5%)对SPI凝胶流变特性和抗氧化活性的影响。结果表明，随着OPC浓度的增加，包埋率增加，荷载量减小，最大荷载量达到35.575 3μg/mg。随着OPC浓度的增加，OPC-SPI凝胶的弹性模量和黏性模量增加，损耗因子随之降低，对频率的依赖性降低；但对凝胶的黏度无显著差异。OPC-SPI对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基以及羟自由基的清除能力优于SPI。OPC能够有效增强SPI的凝胶黏弹性和抗氧化活性，为进一步开发新型抗氧化复合SPI凝胶提供了理论依据。

关键词(KeyWords)： 原花青素;大豆分离蛋白;流变特性;抗氧化活性

Abstract:

Keywords:

基金项目(Foundation): 博士启动基金项目(318051603);; 山东省农业科技园区产业提升工程项目(2019YQ035);; 国家自然科学基金青年基金项目(32101788);; 聊城大学博士科研启动基金项目(318052021)

作者(Author): 李婧御,李元鑫,刘冉,孙永旺,郭尚敬,穆洪静,赵庆奎

参考文献(References)：

• [1]ZHANG X,SONG X,HU X,et al.Health benefits of proanthocyanidins linking with gastrointestinal modulation:an updated review[J].Food Chemistry,2023,404:134596.
• [2]SARKAR P,THIRUMURUGAN K.Modulatory functions of bioactive fruits,vegetables and spices in adipogenesis and angiogenesis[J].Journal of Functional Foods,2019,53:318-336.
• [3]TIE S S,SU W T,CHEN Y N,et al.Dual targeting procyanidin nanoparticles with glutathione response for colitis treatment[J].Chemical Engineering Journal,2022,441:136095.
• [4]QI Q,CHU M,YU X,et al.Anthocyanins and proanthocyanidins:chemical structures,food sources,bioactivities,and product development[J].Food Reviews International,2023,39(7):4581-4609.
• [5]SUN X,WU X,CHEN X,et al.Casein-maltodextrin Maillard conjugates encapsulation enhances the antioxidative potential of proanthocyanidins:an in vitro and in vivo evaluation[J].Food Chemistry,2021,346:128952.
• [6]SHENG K,ZHANG G,KONG X,et al.Encapsulation and characterisation of grape seed proanthocyanidin extract using sodium alginate and different cellulose derivatives[J].International Journal of Food Science & Technology,2021,56(12):6420-6430.
• [7]HUANG Y,LIN J,TANG X,et al.Grape seed proanthocyanidin-loaded gel-like W/O/W emulsion stabilized by genipin-crosslinked alkaline soluble polysaccharides-whey protein isolate conjugates:fabrication,stability,and in vitro digestion[J].International Journal of Biological Macromolecules,2021,186:759-769.
• [8]GUO J,HE Z,WU S,et al.Binding of aroma compounds with soy protein isolate in aqueous model:effect of preheat treatment of soy protein isolate[J].Food Chemistry,2019,290:16-23.
• [9]WANG Z,ZENG L,FU L,et al.Effect of ionic strength on heat-induced gelation behavior of soy protein isolates with ultrasound treatment[J].Molecules,2022,27(23):8221.
• [10]SU Y,DONG Y,NIU F,et al.Study on the gel properties and secondary structure of soybean protein isolate/egg white composite gels[J].European Food Research and Technology,2014,240(2):367-378.
• [11]XIAO Y,LI J,LIU Y,et al.Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose[J].Food Chemistry,2020,324:126876.
• [12]WU M,JIN J,JIN P,et al.Epigallocatechin gallate-β-lactoglobulin nanoparticles improve the antitumor activity of EGCG for inducing cancer cell apoptosis[J].Journal of Functional Foods,2017,39:257-263.
• [13]AO L,LIU P,WU A,et al.Characterization of soybean protein isolate-food polyphenol interaction via virtual screening and experimental studies[J].Foods,2021,10(11):2813.
• [14]HAN L,PENG X,CHENG Y,et al.Effects of catechin types found in tea polyphenols on the structural and functional properties of soybean protein isolate-catechin covalent complexes[J].LWT-Food Science and Technology,2023,173:114336.
• [15]刘冉，曾庆华，王振宇，等.超声波处理对大豆分离蛋白凝胶流变性和凝胶形成的影响[J].食品工业科技，2020,41(21):87-92.
• [16]梅瀚，曹金凤，刘世巍，等.超声辅助提取葡萄籽中原花青素工艺及抗氧化活性研究[J].广东化工，2023,50(5):38-41.
• [17]ZHAO H,CHEN J,HEMAR Y,et al.Improvement of the rheological and textural properties of calcium sulfate-induced soy protein isolate gels by the incorporation of different polysaccharides[J].Food Chemistry,2020,310:125983.
• [18]颜军，苟小军，邹全付，等.分光光度法测定Fenton反应产生的羟基自由基[J].成都大学学报(自然科学版),2009,28(2):91-93,103.
• [19]HU Y,LIANG H,XU W,et al.Synergistic effects of small amounts of konjac glucomannan on functional properties of egg white protein[J].Food Hydrocolloids,2016,52:213-220.
• [20]ZHOU X,CHEN T,LIN H,et al.Physicochemical properties and microstructure of surimi treated with egg white modified by tea polyphenols[J].Food Hydrocolloids,2019,90:82-89.
• [21]李立敏，杨豫菘，成立新，等.茶多酚对羊肉肌原纤维蛋白凝胶特性的影响[J].肉类研究，2020,34(3):8-13.
• [22]FENG J,CAI H,WANG H,et al.Improved oxidative stability of fish oil emulsion by grafted ovalbumin-catechin conjugates[J].Food Chemistry,2018,241:60-69.
• [23]MEDINA I,TOMBO I,SATUE-GRACIA M T,et al.Effects of natural phenolic compounds on the antioxidant activity of lactoferrin in liposomes and oil-in-water emulsions[J].Journal of Agricultural and Food Chemistry,2002,50(8):2392-2399.