周浩,涂芬,付欣,钟清,祝欣然,苏云霞,杨芳

• 1:武汉工程大学环境生态与生物工程学院
• 2:武汉工程大学化工与制药学院

摘要(Abstract)：

板栗壳含有多种多酚类物质,原花青素就是其中一类具有抗氧化性的物质。为了充分利用板栗壳资源,文章研究了光照、温度、pH及金属离子对板栗壳粗提物中原花青素稳定性的影响;利用高效液相色谱-质谱(HPLC-MS)对粗提物中原花青素进行结构表征和种类鉴定;通过建立模拟胃和小肠消化环境的体外消化模型,探究原花青素在人体中的消化情况。结果表明:原花青素对光和热不稳定,当温度高于60℃时,其结构被破坏;原花青素在弱酸及中性溶液中稳定,在金属离子Fe~(3+)、Fe~(2+)、Ba~(2+)存在的条件下被降解。通过HPLC-MS将质荷比及二级质谱碎片离子与已报道文献进行对比,鉴定出粗提物中含有没食子酸、儿茶素、没食子儿茶素和B型原花青素二聚体。体外消化模拟实验表明,原花青素在胃及小肠中的消化率达到18.7%和20.5%。总的来说,板栗壳原花青素是一种可消化的天然抗氧化剂来源,可在各类食品、调味品和食品添加剂领域广泛应用,具有一定的开发价值。

关键词(KeyWords)： 板栗壳;原花青素;稳定性;结构分析;体外消化

Abstract:

Keywords:

基金项目(Foundation): 武汉工程大学校长基金(2018153);; 国家级大学生创新训练项目(201810490015);; 湖北省教育厅科学技术研究重点项目(D20171501)

作者(Author): 周浩,涂芬,付欣,钟清,祝欣然,苏云霞,杨芳

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