高通量测序技术在探索芽菜微生物群落结构中的应用Application of High-Throughput Sequencing Technology in Exploring Microbial Community Structure of Sprouts
王新惠,刘力嘉,丁悦,孙劲松,潘攀,张雅琳,庞紫琳,刘洋
摘要(Abstract)：
文章主要通过高通量测序技术探索市场上不同品牌宜宾芽菜之间细菌菌群结构的差异性。采用Alpha多样性分析和Beta分析，从门水平上对细菌菌群结构分析，发现优势菌门是厚壁菌门(Firmicutes),在3个样本中占比分别为43.77%、50.73%、45.59%;在种的水平上进行分析发现，优势菌种是鼠乳杆菌(Lactobacillus murinus),在3个样本中占比分别为10.51%、9.87%、11.92%。此外，还在芽菜中检测出脑膜炎嗜冷杆菌(Psychrobacter meningitidis)和荧光假单胞菌(Pseudomonas fluorescens)等腐败致病菌。结果表明，3种品牌宜宾芽菜之间细菌菌群结构有一定差异，并且市场上流通芽菜的微生物安全需要改善。
关键词(KeyWords)： 芽菜;高通量测序技术;群落结构;优势菌种
基金项目(Foundation): 四川省科技厅应用基础项目(2018JY0365);; 烹饪科学四川省高等学校重点实验室资助项目(PRKX201804);; 固态发酵资源利用四川省重点实验室开放基金项目资助(2018GTJ007)
作者(Author): 王新惠,刘力嘉,丁悦,孙劲松,潘攀,张雅琳,庞紫琳,刘洋
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