发酵豆酱中ACE抑制肽的制备及分离纯化Preparation,Isolation and Purification of Angiotensin-Converting Enzyme Inhibitory Peptide from Fermented Soybean Paste
李利军,马英辉,卢美欢
摘要(Abstract):
为了获得ACE抑制肽,利用微生物发酵法以大豆为原料制成中国传统豆酱,通过研磨、溶解、超滤后获得了ACE抑制活性最高的肽段区间3~10k,并依次利用DEAE琼脂糖微球a、Sephadex G-75凝胶对该组分进行分离纯化,最后纯化获得组分DGIII的ACE抑制活性最高,达到(98.3±1.24)%,IC50为0.08mg/mL,说明豆酱具有开发高抑制率ACE食品的潜力。
关键词(KeyWords): 豆酱;ACE抑制肽;分离纯化
基金项目(Foundation): 陕西省科技攻关重点项目(2015SF165)
作者(Author): 李利军,马英辉,卢美欢
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