纪凤娣,魏巍,陶汇源,李东,夏蓉,鲁绯

• 1:北京市营养源研究所
• 2:镇江恒顺生物工程有限公司

摘要(Abstract)：

多酚化合物的种类和含量是反映食醋营养功能和品质的重要指标之一。紫外可见分光光度法是目前食品中总多酚含量检测研究中应用最广泛的方法之一。通过试验分析了Folin-Ciocalteu试剂测定食醋总多酚含量方法中样品制备、显色剂用量、显色条件和测量时间等因素对分子吸收光谱的影响,基于此,确定了食醋总多酚含量的测定方法,并对中国四大名醋中总多酚含量进行了分析。以没食子酸为校正标准品,配制1%的食醋样品水溶液,取1 mL于10 mL容量瓶中,于8 min内加入0.5 mL Folin-Ciocalteu试剂和1.5 mL 20%的碳酸钠溶液,暗处显色2 h后,在20 min内于760 nm测量其吸光度值。方法验证结果表明,没食子酸标准溶液在2.0～10.0μg/mL范围内线性关系良好,线性回归方程为Y=0.1148X-0.2087,相关系数R~2=0.9992,平均回收率为97.2%,RSD为1.37%。该方法操作简易、检测成本低、重复性良好、测定精度较高,可以为我国食醋中总多酚含量测定行业标准的建立提供有效的技术参考。

关键词(KeyWords)： 食醋;总多酚;Folin-Ciocalteu试剂;分光光度法

Abstract:

Keywords:

基金项目(Foundation): 2018年江苏省重点研发专项(BE2018371);; 2017年“金山英才”镇江制造2025领军人才计划;; 镇江市重点研发计划项目(NY2019019)

作者(Author): 纪凤娣,魏巍,陶汇源,李东,夏蓉,鲁绯

参考文献(References)：

• [1]FARIAS D P,NERI-NUMA I A,DE ARAúJO F F,et al.A critical review of some fruit trees from the Myrtaceae family as promising sources for food applications with functional claims[J].Food Chemistry,2020,306:125630．
• [2]ADDEPALLI V,SURYAVANSHI S V.Catechin attenuates diabetic autonomic neuropathy in streptozotocin induced diabetic rats[J].Biomedicine&Pharmacotherapy,2018,108:1517-1523．
• [3]AGUNLOYE O M,OBOH G,ADEMILUYI A O,et al.Cardio-protective and antioxidant properties of caffeic acid and chlorogenic acid:mechanistic role of angiotensin converting enzyme,cholinesterase and arginase activities in cyclosporine induced hypertensive rats[J].Biomedicine&Pharmacotherapy,2019,109:450-458．
• [4]HUANG B,LIU J,MA D,et al.Myricetin prevents dopaminergic neurons from undergoing neuroin flammation-mediated degeneration in a lipopolysaccharide-induced Parkinson's disease model[J].Journal of Functional Foods,2018,45:452-461．
• [5]ALI F,RAHUL,JYOTI S,et al.Therapeutic potential of luteolin in transgenic Drosophila model of Alzheimer's disease[J].Neuroscience Letters,2019,692:90-99．
• [6]程光胜．山西老陈醋[M]．南京：南京出版传媒集团，南京出版社，2015:20．
• [7]JOHNSTON C S,QUAGLIANO S,WHITE S.Vinegar ingestion at mealtime reduced fasting blood glucose concentrations in healthy adults at risk for type 2diabetes[J].Journal of Functional Foods,2013,5(4):2007-2011．
• [8]LIU J,GAN J,SATORU N,et al.Cellular uptake and trans-enterocyte transport of phenolics bound to vinegar melanoidins[J].JournalofFunctionalFoods,2017,37:632-640．
• [9]LIU J,GAN J,YU Y,et al.Effect of laboratory-scale decoction on the antioxidative activity of Zhenjiang aromatic vinegar:the contribution of melanoidins[J].Journal of Functional Foods,2016,21:75-86．
• [10]CARVALHO D G,RANZAN L,TRIERWEILER L F.Determination of the concentration of total phenolic compounds in aged cacha9a using two-dimensional fluorescence and mid-infrared spectroscopy[J].Food Chemistry,2020,329:127142．
• [11]KRSTONO?I?M A,HOGERVORST J C,MIKULI?M,et al.Development of HPLC method for determination of phenolic compounds on a core shell column by direct injection of wine samples[J].Acta Chromatographica,2020,32(2):134-138．
• [12]POLUMACKANYCZ M,SLEDZINSKI T,GOYKE E,et al.A comparative study on the phenolic composition and biological activities of Morus alba L.commercial samples[J].Molecules,2019,24(17):3082-3100．
• [13]ARRIBAS A S,MARTíNEZ-FERNáNDEZ M,MORENOM,et al.Classification of Spanish white wines using their electrophoretic profiles obtained by capillary zone electrophoresis with amperometric detection[J].Electrophoresis,2014,35(11):1693-1700．
• [14]DIAS F S,KLASSEN A,TAVARES M F M,et al.Fast determination of phenolic compounds in Brazilian wines from Vale do S2o Francisco Region by CE[J].Chromatographia,2013,76(9-10):559-563．
• [15]ZHAO H,ZHU M,WANG K,et al.Identification and quantitation of bioactive components from honeycomb(Nidus vespae)[J].Food Chemistry,2019,314:127142．
• [16]SEZGIN S,DALAR A,UZUN Y.Determination of antioxidant activities and chemical composition of sequential fractions of five edible mushrooms from Turkey[J].Journal Food Science Technology,2020,57:1866-1876．
• [17]SINGLETON V L,ROSSI J A.Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagent[J].American Journal of Enology and Viticulture,1965,16:144-158．
• [18]SINGLETON V L,ORTHOFER R,LAMUELA-RAVENTóSR M.Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent[J].Methods in Enzymology,1999,299:152-178．
• [19]宋烨，翟衡，杜远鹏，等．苹果加工品种多酚提取物的抗氧化效果分析[J]．果树学报，2006,23(6):793-797．
• [20]李利敏，沈建福，吴晓琴．8种油茶蒲提取物中活性物质含量及其抗氧化能力的比较研究[J]．中国粮油学报，2013,28(1):41-47．
• [21]靳磊，张延龙，牛立新，等．3种百合鳞茎中多酚类物质的抗氧化活性分析[J]．西北植物学报，2014,34(5):995-1001.