胡珺,王常高,林建国,杜馨,蔡俊

• 1:湖北工业大学发酵工程教育部重点实验室工业发酵湖北省协同创新中心

摘要(Abstract)：

研究采用响应面法对假单胞菌HFE733产脂肪酶的发酵培养基进行了优化。在前期运用单因素试验优化的基础上,利用Plackett-Burman试验设计筛选出培养基组分中影响脂肪酶活力的2个显著性因素:酵母膏和橄榄油。运用单因素试验研究非显著因素的最低添加量来降低生产成本,然后运用最陡爬坡试验找到中心复合试验的中心点,最后利用中心复合设计确定显著性因素之间的交互作用及最佳组成。结果表明:最佳培养基组分为酵母膏34g/L、橄榄油5.92 mL/L、蔗糖12.5g/L、CuSO_4·5H_2O 0.4g/L、MnSO_4·H_2O 0.2g/L,在此条件下脂肪酶酶活可达到9.27U/mL,与模型预测值相近,且与单因素优化后的酶活(4.53U/mL)相比,提高了104.64%。

关键词(KeyWords)： 假单胞菌;脂肪酶;响应面法;培养基优化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31401807)

作者(Author): 胡珺,王常高,林建国,杜馨,蔡俊

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