基于响应面法与电子鼻技术优化发酵型滇橄榄辣椒酱的制备工艺Optimization of Preparation Process of Fermented Phyllanthus emblica Chili Sauce Based on Response Surface Method and Electronic Nose Technology
刘猛,吕文浩,王茂森,李祥,连泽源,张璐
摘要(Abstract):
滇橄榄富含多酚、黄酮、鞣花酸类物质,具有开发为功能性食品和发酵调味品的潜力。该研究针对发酵型滇橄榄辣椒酱的风味和品质优化问题,系统探讨了发酵剂接种量、发酵温度、发酵时间对产品品质的影响。通过单因素试验筛选关键因素,并基于感官评分进行三因素三水平响应面优化试验,同时结合电子鼻检测、理化指标测定和微生物指标分析,对产品的风味特征和安全性进行综合评价。结果表明,最佳发酵条件为发酵剂接种量2%、发酵时间6 d、发酵温度20℃,此条件下感官评分达到95.5分,总酸含量为1.13%。此时发酵型滇橄榄辣椒酱口感细腻、风味独特、酱体较均匀,产品的理化指标、微生物指标均符合国家标准。该研究明确了发酵型滇橄榄辣椒酱的最佳发酵工艺,并揭示了关键工艺因素对风味和品质的影响机制,为滇橄榄的深加工开发提供了科学依据。
关键词(KeyWords): 滇橄榄;发酵;辣椒酱;电子鼻;响应面法
基金项目(Foundation): 山西省高等学校科技创新项目(2022L562);; 吕梁市重点研发项目(2025NY24,2023NYYF13)
作者(Author): 刘猛,吕文浩,王茂森,李祥,连泽源,张璐
参考文献(References):
- [1]杨丽梅,许秀玲,张世宇.滇橄榄野生资源改造利用[J].云南农业,2025,39(6):50-51.
- [2]刘祚祚,赵雪竹,蒲慧,等.滇橄榄不同采摘期果实品质的变化规律[J].食品工业科技,2022,43(6):293-301.
- [3]Tiwana G,Cock I E,Cheesman M J.Phytochemistry,antibacterial and toxicity properties of Phyllanthus emblica Linn fruit extracts against gastrointestinal pathogens and their interactions with antibiotics[J].Pharmacological Research-Natural Products,2025,9:100386.
- [4]Pu Tianlei,Yuan Jiamin,Qu Wenlin,et al.Selection and validation of reference candidate genes for qRT-PCR analysis in the developing fruit of Phyllanthus emblica L.[J].Horticulturae,2025,11(9):1054.
- [5]姜北.白族药用植物——滇橄榄[J].大理大学学报,2020,5(4):102.
- [6]常庆.滇橄榄多糖提取纯化、结构表征及生物活性研究[D].昆明:昆明医科大学,2022.
- [7]Ma Qinge,Wang Lei,Liu Ronghua,et al.Phyllanthus emblica Linn:a comprehensive review of botany,traditional uses,phytonutrients,health benefits,quality markers,and applications[J].Food Chemistry,2024,446:138891.
- [8]Fatima E,Arooj I,Javeed M,et al.Green synthesis,characterization and applications of Phyllanthus emblica fruit extract mediated chromium oxide nanoparticles[J].Discover Nano,2024,19(1):68.
- [9]Singh D,Verma A,Anwar F,et al.Biogenic synthesis of ferric nanoparticles of Phyllanthus emblica exhibited breast cancer effect via alteration of mTOR/Maf-1/PTEN signaling pathway[J].Biocatalysis and Agricultural Biotechnology,2024,56:103007.
- [10]Gai Zhenyu,Wang Ying,Xian Minghua,et al.Comprehensive assessment of the extraction of Phyllanthus emblica L.based on the G1-entropy method and response surface methodology[J].Journal of Applied Research on Medicinal and Aromatic Plants,2024,39:100536.
- [11]杨顺楷,黄德英,杨亚力.滇橄榄汁酶解产物没食子酸的TLC和GC测定[J].四川食品与发酵,2008(3):51-53.
- [12]赵承鑫.凯里白酸汤发酵工艺优化及酸汤饮料开发[D].成都:四川大学,2022.
- [13]骆扬,张福显.滇橄榄梨膏软糖的研制[J].文山学院学报,2023,36(5):19-24.
- [14]李瑞婷,单林鲜,倪庆,等.基于电子舌技术的滇橄榄汁滋味品质评价[J].现代食品科技,2022,38(6):267-274.
- [15]骆扬,王永健.滇橄榄西番莲复合果汁饮料的研制[J].文山学院学报,2016,29(6):39-42.
- [16]张郁松.植物乳杆菌强化发酵辣椒酱工艺[J].食品工业,2024,45(5):20-22.
- [17]Narayanan M,Suresh K,Al Obaid S,et al.Statistical optimized production of phytase from Hanseniaspora guilliermondii S1 and studies on purification,homology modelling and growth promotion effect[J].Environmental Research,2024,252:118898.
- [18]王思捷,李昶甫,袁灿,等.基于GC-IMS、电子鼻和电子舌联用技术分析不同加工工艺辣椒对烧椒酱风味特性的影响[J].中国调味品,2025,50(8):184-191,202.
- [19]白家瑞,迟明,刘洋,等.响应面法优化羊肚菌牦牛肉酱配方研究[J].高原农业,2025,9(3):411-420.
- [20]张翼,赵炜,周帼萍.复合调味酱制品的污染微生物分析[J].中国调味品,2016,41(3):53-56.
- [21]林焕志,周森林,陈祖明,等.响应面法优化鹿茸菇即食酱的制作工艺[J].农产品加工,2025(18):40-46.
- [22]包宇畅,林婉佳,江仪婷.响应面法优化老香黄卡仕达酱的工艺[J].食品工业,2025,46(3):14-18.