响应面分析法优化南美白对虾的酶解工艺研究Optimization of Enzymatic Hydrolysis of South American White Shrimp by Response Surface Analysis
窦银花,田怀香,宋诗清,易封萍,肖作兵
摘要(Abstract):
利用响应面法对南美白对虾的酶解工艺进行优化。以南美白对虾为原料,采用碱性蛋白酶和风味蛋白酶分步酶解的方法,以蛋白水解度(DH)为衡量指标。结合单因素分析考察底物浓度、风味蛋白酶的添加量和风味蛋白酶的反应时间三因素三水平的中心组合实验设计,得出一个多元线性回归方程模型。在分析各个因素的显著性和交互作用后,得出南美白对虾酶解工艺的最佳条件:底物浓度15%,风味蛋白酶添加量0.8%,风味蛋白酶酶解时间3.0h,水解度高达47.19%。
关键词(KeyWords): 酶解;南美白对虾;响应面分析
基金项目(Foundation): “十二五”国家科技支撑计划“食品香料香精制备关键技术研究及产业化”(2011BAD23B01)
作者(Author): 窦银花,田怀香,宋诗清,易封萍,肖作兵
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