超声波辅助热加工泡椒猪肝中优势腐败菌的分离鉴定及抑菌试验Isolation,Identification and Bacteriostatic Test of Dominant Spoilage Bacteria in Pig Liver with Pickled Pepper by Ultrasonic-assisted Hot Processing
任政伟,王艺华,刘春燕,丁捷,刘浩宇,陈美玲
摘要(Abstract):
课题组从37℃下存放的超声波辅助热加工泡椒猪肝中分离筛选出5株优势腐败菌,对其进行菌落形态、革兰氏染色、生理生化试验以及16SrDNA片段扩增及序列分析技术分析,最终确定为多粘类芽孢杆菌(MF001284.1)、莓实假单胞菌(JX867753.1)、枯草芽孢杆菌(MF146958.1)、枯草芽孢杆菌(CP021169.1)、枯草芽孢杆菌属(KY228939.1)。通过固体平板扩散法从4种天然抑菌物质中筛选出2种抑菌效果明显的抑菌剂:乳酸和醋酸,其最低抑制浓度分别为2.0%、2.0%。
关键词(KeyWords): 乳酸;醋酸;超声波辅助热杀菌;泡椒猪肝;抑菌效果
基金项目(Foundation): 四川省教育厅自然科学一般项目(17ZB0326)
作者(Author): 任政伟,王艺华,刘春燕,丁捷,刘浩宇,陈美玲
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