王琪,杨淑艺,刘鹏帆,蔡瑾,秦文君

• 1:山西大学生命科学学院
• 2:江西李渡酒业有限公司
• 3:山西大学应用化学研究所
• 4:山西省中医药研究院营养科

摘要(Abstract)：

从晋西北酸粥发酵液中分离到一株短乳杆菌M-10,发现其具有耐酸、耐胆盐、抗氧化、抗菌、降胆固醇等多种益生特性。为了更好地应用该菌株，该研究评价了其体外和体内的安全性，包括溶血活性检测、明胶液化能力测定、硝基还原酶活性测定、吲哚和生物胺生成能力测定、SD大鼠急性毒性和亚急性毒性实验。经检测发现，短乳杆菌M-10缺乏溶血活性，不能液化明胶，缺乏硝基还原酶活性，不会生成吲哚，不能产生生物胺，包括酪胺、组胺、尸胺、腐胺等；该菌对SD大鼠的一般体征和血常规指标均无明显不良影响。以上研究结果表明，短乳杆菌M-10具有很好的安全性。该研究可为后续短乳杆菌M-10作为发酵剂或益生菌的开发奠定基础。

关键词(KeyWords)： 晋西北酸粥;短乳杆菌;溶血活性;明胶液化活性;硝基还原酶活性;吲哚测定;生物胺测定;急毒亚急毒实验

Abstract:

Keywords:

基金项目(Foundation): 山西省自然科学研究面上项目(20210302123464)

作者(Author): 王琪,杨淑艺,刘鹏帆,蔡瑾,秦文君

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