班雯婷,胡彪,康佩姿,刘娟

• 1:广东省科学院生物工程研究所

摘要(Abstract)：

以灵芝、平菇、秀珍菇、茶树菇制备真菌诱导子和金属离子、硝酸铈、氯化镉、茉莉酸甲酯制备化学诱导子，研究了其对法夫酵母生长和新科斯糖合成的影响。结果表明，真菌诱导子对法夫酵母菌体生长、新科斯糖合成有显著促进作用，化学诱导子对生长作用不一致但能促进新科斯糖的合成。灵芝、平菇、秀珍菇、茶树菇诱导子在添加量分别为10,20,5,10 mg/L时生物量最高，分别比对照组提高了12.50%、12.11%、7.81%和7.99%,灵芝、平菇、秀珍菇和茶树菇制备的诱导子在添加量分别为10,30,10,20 mg/L时新科斯糖含量最高，分别比对照提高了11.85%、15.08%、19.03%和9.87%;Mn~(2+)和Zn~(2+)能明显促进细胞生长，增强果糖基转移酶的活性，最佳浓度下，新科斯糖积累量增加了9.56%和5.18%,硝酸铈、氯化镉和茉莉酸甲酯诱导子在添加量分别为0.16,0.12,0.08 mg/L时新科斯糖含量最高，分别比对照组提高了9.97%、14.08%和5.91%。

关键词(KeyWords)： 法夫酵母;新科斯糖;真菌诱导子;化学诱导子;条件优化

Abstract:

Keywords:

基金项目(Foundation): 广东省科学院专项资金项目(2019GDASYL-20200102009);广东省科学院专项资金项目(2019GDASYL-20200102010);广东省科学院专项资金项目(2019GDASYL-0105028)

作者(Author): 班雯婷,胡彪,康佩姿,刘娟

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