闫佳佳,万璐,姜硕,许哲祥,郑春英

• 1:黑龙江大学农业微生物技术教育部工程研究中心
• 2:黑龙江大学生命科学学院黑龙江省普通高校微生物重点实验室

摘要(Abstract)：

采用TLC法,以7种黄酮成分为对照,分析黑龙江汉麻叶中黄酮成分;同时采用HPLC法对汉麻叶中所含黄酮成分加以确认;并采用抑菌活性实验观察汉麻叶的抑菌作用。结果表明,在汉麻叶中含有芦丁、槲皮素、槲皮素-3-O-α-L-鼠李糖苷、异牡荆素、牡荆素和木犀草素等黄酮成分;抑菌活性实验结果表明,汉麻叶对10种致病菌均有较好的抑制作用,尤其对3种食源性致病菌的抑菌效果较为明显。上述研究为开发汉麻叶黄酮成分奠定了基础。

关键词(KeyWords)： 汉麻叶;黄酮;HPLC;抑菌活性

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省现代农业产业技术协同创新体系项目(YYM19STX-1)

作者(Author): 闫佳佳,万璐,姜硕,许哲祥,郑春英

参考文献(References)：

• [1]国家药典委员会．中华人民共和国药典2015年版(一部)[S]．北京：中国医药科技出版社，2015:80．
• [2]Nong Y F,Zhu D S,Li J.Establishment of tissue culture regeneration system in Bama hemp(Cannabis sativa L.)[J].Agricultural Biotechnology,2019,8(6):1-3．
• [3]Jang E Y,Kim H J,Jang S J,et al.Concentrations of THC,CBD,and CBN in commercial hemp seeds and hempseed oil sold in Korea[J].Forensic Science International,2020,306:1-8．
• [4]Nora Pap,Leena Hamberg,Juha-Matti Pihlava,et al.Impact of enzymatic hydrolysis on the nutrients,phytochemicals and sensory properties of oil hemp seed cake(Cannabis sativa L.FINOLA variety)[J].Food Chemistry,2020,320:1-10．
• [5]Portp C D,Decorti D,Tubaro F.Fatty acid composition and oxidation stability of hemp(Cannabis sativa L.)seed oil extracted by supercritical carbon dioxide[J].IndustrialCrops and Products,2012,36:401-404．
• [6]Nalli Yedukondalu,Arora Palak,Riyaz-Ul-Hassan,et al.Chemical investigation of Cannabis sativa leading to the discovery of a prenylspirodinone with anti-microbial potential[J].Tetrahedron Letters,2018,59:2470-2472．
• [7]Sara A B,Marika P,Simone T,et al.Cannabis sativa:a comprehensive ethnopharmacological review of a medicinal plant with a long history[J].Journal of Ethnopharmacology,2018,227:300-315．
• [8]Radwan M M,Elsohly M A,Slade D,et al.Non-cannabinoid constituents from a high potency Cannabis sativa variety[J].Phytochemistry,2008,69:2627-2633．
• [9]Ibrahim A K,Radwan M M,Ahmed S A,et al.Microbial metabolism of cannflavin A and B isolated from Cannabis sativa[J].Phytochemistry,2010,71:1014-1019．
• [10]李杰，普玲玲，王李雪，等．花椒黄酮提取工艺及体外抗氧化活性研究[J]．中国调味品，2019,44(9):94-100．
• [11]罗晓，呼海涛，龚海燕，等．石香薷总黄酮调味品的制备[J]．中国调味品，2019,43(1):118-121．
• [12]Roberta A,Lucia C,Silvia T,et al.Valorisation of hemp inflorescence after seed harvest:cultivation site and harvest time influence agronomic characteristics and essential oil yield and composition[J].Industrial Crops&Products,2019,139:1-8．
• [13]Muhammad T,Durre S.GCMS analysis of Cannabis sativa L.from four different areas of Pakistan[J].Egyptian Journal of Forensic Sciences,2015,5:114-125．
• [14]高娟娟，贾丽艳，田宇敏，等．枯草芽孢杆菌CGMCC6624对抗生素耐受性的研究[J]．中国调味品，2019,44(3):31-35．
• [15]朱奇奇，张杰，谢平，等．八角多酚提取物对植物病原真菌的抑制作用研究[J]．中国调味品，2017,42(5):5-8．
• [16]成亮，孔德云，胡光．工业大麻研究．I．甲醇提取物的石油醚萃取和正丁醇萃取部分的化学成分[J]．中国医药工业杂志，2008,39(1):18-21．
• [17]Delgado-Povedano M M,Sánchez-Carnerero Callado C,Priego-Capote F,et al.Untargeted characterization of extracts fromCannabis sativa L.cultivars by gas and liquid chromatography coupled to mass spectrometry in high resolution mode[J].Talanta,2020,208:1-10．
• [18]赵璇．大蒜水提物抑菌作用的研究[J]．中国调味品，2019,44(11):198-200.