吴建楠,陆龙波,高利平,王金玲

• 1:东北林业大学生命科学学院
• 2:黑龙江省森林食品资源利用重点实验室

摘要(Abstract)：

现阶段国内外生物合成树莓酮的研究主要以基因工程的手段为主，未发现能直接高产树莓酮的菌株，且发酵使用的原料通常价格较高，不具备大规模生产的条件。该研究以陆生伊萨酵母WJL-G4为发酵菌种，以树莓叶提取液为发酵原料，添加碳源、氮源、无机盐和部分树莓酮上游产物，通过单因素试验、最陡爬坡试验和响应面试验，研究培养基成分对树莓酮产量的影响。确定最佳摇瓶发酵培养基配方为向30 mL树莓叶提取液中加入1.30 g/L对香豆酸、0.80 g/L肉桂酸和1.75 g/L苯丙氨酸，此时测得树莓酮产量平均值为139.02 mg/L。该试验使用陆生伊萨酵母WJL-G4生产树莓酮，操作简单，成本低廉，具有大规模工业生产的可能性，对树莓酮生物合成的发展具有重要参考价值。

关键词(KeyWords)： 陆生伊萨酵母WJL-G4;树莓酮;响应面;培养基优化;树莓叶

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2022YFD1600500);; 中央高校基本科研业务费专项(2572022DX05)

作者(Author): 吴建楠,陆龙波,高利平,王金玲

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