大蒜-麻椒-芹菜复合植物添加剂替代亚硝酸盐改善肉肠贮藏品质的协同机制Synergistic Mechanism of Replacing Nitrite with Garlic-Pepper-Celery Composite Plant Additive in Improving the Storage Quality of Meat Sausage
齐文,滕思雨,杨星耀,杜昱宏,孙莹,李越
摘要(Abstract):
针对肉制品中亚硝酸盐潜在的健康风险,以开发高效、安全的复合植物添加剂为目标,系统研究了大蒜粉、麻椒粉与芹菜冻干粉复配对肉肠贮藏品质的协同改善作用及机制。首先,通过正交试验优化得到复合添加剂最佳配比为大蒜粉添加量12 g/kg、麻椒粉添加量10 g/kg、芹菜冻干粉添加量15 g/kg。在此基础上进行为期21 d的4℃贮藏试验,结果表明试验组(复合植物添加剂)的水分损失最少,菌落总数与乳酸菌数增长最缓慢,且对脂质氧化的抑制作用突出。电子鼻结合PCA显示,其风味轮廓在贮藏期间高度稳定,与阳性对照组重叠且显著优于各缺失组。该体系还展现出独特的安全性优势,其亚硝酸盐峰值含量(35.63 mg/kg)和终点含量(19.98 mg/kg)均低于国家限量标准。缺失组试验进一步证实了大蒜素(核心抗菌)、麻椒多酚(辅助抗氧化)以及芹菜来源的硝酸盐和抗氧化物质(发色与稳定关键组分)在三者协同中不可或缺的作用。综上,该复合植物添加剂通过多组分协同,在不依赖合成亚硝酸钠的前提下,全面实现了对其发色、抑菌、抗氧化、综合品质保持功能的等效替代,为开发健康、安全的肉制品提供了可靠的理论依据与可行的技术方案。
关键词(KeyWords): 天然添加剂;亚硝酸盐;肉肠;贮藏品质;正交试验
基金项目(Foundation): 2024年度黑龙江省省属本科高校基本科研业务费项目(2024-KYYWF-0984)
作者(Author): 齐文,滕思雨,杨星耀,杜昱宏,孙莹,李越
参考文献(References):
- [1]潘卓官,吴志钦,周斯南,等.不同处理方式对金线鱼肉肠冷藏期间品质特性的影响[J].食品科学,2024,45(19):172-181.
- [2]Flores M,Toldrá F.Chemistry,safety,and regulatory considerations in the use of nitrite and nitrate from natural origin in meat products—invited review[J].Meat Science,2021,171:108272.
- [3]何丹.天然植物提取物替代亚硝酸盐对传统肉制品产品特性的影响研究[D].成都:成都大学,2020.
- [4]Onder S,Periz A D,Ulusoy S,et al.Chemical composition and biological activities of essential oils of seven cultivated Apiaceae species[J].Scientific Reports,2024,14(1):10052.
- [5]赵丽梅.大蒜素提取工艺优化及清除PTIO自由基的研究[D].衡阳:南华大学,2023.
- [6]李军,汤莉,黄立,等.大蒜素对青脚麻鸡生长性能、免疫功能、血清生化指标的影响[J].饲料研究,2023,46(20):41-46.
- [7]Dragan M,Christine M,Aedin C,et al.Interindividual variability in biomarkers of cardiometabolic health after consumption of major plant-food bioactive compounds and the determinants involved[J].Advances in Nutrition,2017,8(4):558-570.
- [8]Saini A,Saini V,Deswal G,et al.Harnessing therapeutic potential of allicin against cancer:an exploratory review[J].Anti-Cancer Agents in Medicinal Chemistry,2025,25(9):589-602.
- [9]Zhao Liangyi,Lu Yixia,Zhao Yaqin,et al.H2S treatment alleviates postharvest chilling injury in green pepper by regulating antioxidant capacity,osmotic regulation and lipid metabolism to maintain cell membrane integrity[J].Food Chemistry,2025,492(2):145428.
- [10]Li Kelin,Uyanga V A,Wang Xiaojuan,et al.Allicin promotes glucose uptake by activating AMPK through CSE/H2S-induced S-sulfhydration in a muscle-fiber dependent way in broiler chickens[J].Molecular Nutrition & Food Research,2024,68(5):15-25.
- [11]Vichaibun V,Sophonnithiprasert T.Antioxidant,α-amylase,and α-glucosidase inhibitory activities of onion (Allium cepa) and shallot (Allium oscalonicum L.) in food ingredients[J].Acta Alimentaria,2024,53(2):214-220.
- [12]Dursun A,Guler Z.Postmortem protein phosphorylation in garlic-treated meat during refrigerated storage:glycolytic and proteolytic changes[J].Food Research International,2025,208(5):116236.
- [13]Ivane N M A,Haruna S A,Wang Wenxiu,et al.Characterization,antioxidant activity and potential application fractionalized Szechuan pepper on fresh beef meat as natural preservative[J].Meat Science,2024,208:109383.
- [14]Jang J W,Lim C B,Ha J H,et al.Comparative analysis on Micelle herbal extract and Coenzyme Q10 supplementation on growth performance,nutrient digestibility,gas emission,and blood profile in grow-finishing pigs[J].Journal of Animal Science,2025,103:279-287.
- [15]杨秀芳,惠昱昱,龙园园,等.花椒籽中水不溶性膳食纤维提取工艺及其特性[J].陕西科技大学学报,2018,36(3):63-67.
- [16]陈志宏,陈静,张汆,等.青、红花椒提取物对腊肠质量特性的影响[J].中国调味品,2018,43(11):48-54.
- [17]盛文盼,杨立军,刘楠,等.影响芹菜植物空心胶囊薄膜力学性能的因素研究[J].陕西科技大学学报,2018,36(5):129-133,140.
- [18]张雪松,高慧宇,王国栋,等.芹菜粉中总膳食纤维标准物质的研制[J].食品安全质量检测学报,2020,11(18):6386-6390.
- [19]张诗雯.芹菜对鱼糜制品凝胶品质及体外消化特性的影响[D].锦州:渤海大学,2019.
- [20]熊绍风.芹菜素通过激活PI3K-Akt-mTOR通路抑制自噬促进白色脂肪棕色化[D].南昌:南昌大学,2020.
- [21]李昌文,张丽华,纵伟,等.芹菜渣水溶性膳食纤维的抗氧化活性研究[J].食品工业,2016,37(9):63-66.
- [22]游艳芝,苏萃扬,张亮,等.膳食纤维不同改性技术研究进展[J].食品科学,2025,46(13):406-416.
- [23]Wu Liu,Zhao Linyu,Tao Yingmei,et al.Improving the aroma profile of inoculated fermented sausages by constructing a synthetic core microbial community[J].Journal of Food Science,2023,88(11):4388-4402.
- [24]Cheng Jingrong,Chen Peiyu,Liu Xueming,et al.Oxidation and nitrosation in Cantonese sausage during processing and storage—the impact of adding Prunus mume polyphenol[J].Food Control,2025,167:110789.
- [25]杨楠.大蒜活性成分的提取分离及其抗菌、抗生物被膜活性的研究[D].南宁:广西中医药大学,2023.
- [26]袁惠萍,赵钜阳,崔建涛,等.儿茶素对大豆拉丝蛋白素干贝贮藏品质的影响[J].中国调味品,2025,50(3):115-119.
- [27]代安华.大蒜中大蒜素及其他活性成分的生物学功能及健康效应[J].中国饲料,2020(16):5-8.
- [28]高雪琴,刘燕,冯春梅,等.复合蔬菜汁部分替代亚硝酸盐对鲜烧鸡保质期的影响[J].中国调味品,2024,49(4):96-103.
- [29]Yi Lmaz O Z F,Kaya M,Kaban G.Using celery powder in a semi-dry fermented sausage 'heat-treated Sucuk':nitrosamine formation,lipid oxidation,and volatile compounds[J].Foods,2024,13(20):3306.
- [30]Xiao Yue,Zhang Shiyao,Liu Zhijia,et al.Effect of inoculating Pichia spp.starters on flavor formation of fermented chili pepper:metabolomics and genomics approaches[J].Food Research International,2023,173:113397.
- [31]Mann T F,Reagan J O,Lillard D A,et al.Effects of phosphate in combination with nitrite or Maillard reaction products upon warmed-over flavor in precooked,restructured beef chuck roasts[J].Journal of Food Science,2010,54(6):1431-1433.
- [32]Mielnik M B,Aaby K,Skrede G.Commercial antioxidants control lipid oxidation in mechanically deboned turkey meat[J].Meat Science,2003,65(3):1147-1155.