西番莲叶多糖提取工艺的优化及其体外抗氧化活性研究Study on Optimization of Extraction Process of Polysaccharides from Passiflora edulis L. Leaves and Their Antioxidant Activity in Vitro
李霞,胡楠,陈齐琳,王一,李水珍,徐思建,崔萌佳,李静
摘要(Abstract):
采用微波辅助水提西番莲叶多糖,在单因素料液比、微波功率及提取时间的基础上,结合响应面优化西番莲叶多糖的提取工艺,并分析其体外抗氧化活性。结果表明,西番莲叶多糖提取的最佳工艺条件为:料液比1∶20(g/mL),提取功率495 W,提取时间3min,多糖提取率为11.24%±0.50%,与模型预测值相符。西番莲叶多糖浓度为1.0mg/mL时,其DPPH·清除率、还原性能力、·OH清除率以及超氧阴离子自由基清除率分别为88.79%、0.567、42.58%和12.31%。
关键词(KeyWords): 西番莲叶;多糖;响应面;抗氧化活性
基金项目(Foundation): 国家自然科学基金地区基金(31860251)
作者(Author): 李霞,胡楠,陈齐琳,王一,李水珍,徐思建,崔萌佳,李静
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