李锦清,綦艳,郦明浩,邱启东

• 1:广东产品质量监督检验研究院

摘要(Abstract)：

建立固相萃取-高效液相色谱-串联质谱/质谱法(SPE-HPLC-MS/MS)测定酱油中4-甲基咪唑的分析方法。试样经水稀释提取,LC-SCX固相萃取柱净化后,以乙腈和5mmol/L乙酸铵溶液(含0.1%甲酸,V/V)作为流动相,C18色谱柱分离,采用电喷雾离子源,正离子扫描多反应监测(MRM)模式进行检测。4-甲基咪唑在5~500μg/L浓度范围内呈良好线性关系,相关系数(r2)大于0.99,加标回收率为83.6%~95.1%,相对标准偏差为7.3%~8.2%,方法检出限为5μg/kg,定量限为10μg/kg。方法简单快速、准确灵敏,适用于酱油中4-甲基咪唑的测定。

关键词(KeyWords)： 4-甲基咪唑;高效液相色谱-串联质谱法;固相萃取;酱油

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李锦清,綦艳,郦明浩,邱启东

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