储文,田美,黄佃宇,马卫兴

• 1:江苏海洋大学药学院
• 2:江苏海洋大学生物医药产业学院
• 3:江苏省海洋药物筛选重点实验室

摘要(Abstract)：

基于甘草酸二铵与三苯甲烷类阳离子染料乙基紫发生离子缔合反应，首次提出离子缔合褪色反应分光光度法间接测定甜味剂甘草酸含量的新方法。在pH 8.6的Clark-Lubs缓冲溶液中，甘草酸二铵与乙基紫发生离子缔合反应，生成的离子缔合物使溶液显著褪色，褪色的最大吸收波长在594 nm处，据此建立了间接测定甘草酸的分析方法。结果显示，甘草酸二铵的浓度在3.00～12.00 mg/L之间与吸光度差值ΔA呈良好的线性关系，相关系数为0.999 5,检出限为0.46 mg/L,表观摩尔吸光系数为3.20×10~(4 )L/(mol·cm)。方法成功用于市售凉茶样品的含量测定，加标平均回收率为97.11%～101.0%,测定值的相对标准偏差RSD为0.59%～3.23%(n=5)。该法灵敏度高、专属性强、省时简便，可用于实际样品分析。

关键词(KeyWords)： 甘草酸;乙基紫;褪色分光光度法;含量测定;离子缔合反应

Abstract:

Keywords:

基金项目(Foundation): 2021年江苏省首批一流本科课程

作者(Author): 储文,田美,黄佃宇,马卫兴

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