固相萃取结合薄层色谱法测定蜂蜜中高果糖淀粉糖浆Determination of High Fructose Starch Syrup in Honey by Solid-phase Extraction Combined with Thin-layer Chromatography
马凯,杨昌彪,黄永桥,吴新文,简银池
摘要(Abstract):
建立了蜂蜜中高果糖淀粉糖浆的固相萃取结合薄层色谱的测定方法。该研究在GB/T 18932.2-2002方法的基础上,采用固相萃取小柱代替活性炭玻璃层析柱,结合固相萃取装置,对蜂蜜样品中的高分子糖进行分离、富集,再经薄层色谱法检测来判定蜂蜜中高果糖淀粉糖浆的存在。该方法可以进行批量样品的同时处理,减少了净化填料和试剂使用量,缩短了前处理时间,降低了检测成本,提高了检测效率,与标准方法的实验结果一致,表明该方法适用于对蜂蜜中掺有的高果糖淀粉糖浆进行定性判断。
关键词(KeyWords): 蜂蜜;固相萃取;薄层色谱;高果糖淀粉糖浆
基金项目(Foundation): 贵阳国家高新技术产业开发区科技计划项目(GXYF-2017-004);; 贵州省高层次创新型人才培养“千”层次
作者(Author): 马凯,杨昌彪,黄永桥,吴新文,简银池
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