Bacillus amyloliquefaciens SY07发酵绿豆的体外降血糖活性及风味成分分析Hypoglycemic Activity in Vitro and Flavor Component Analysis of Mung Beans Fermented by Bacillus amyloliquefaciens SY07
赵雪如,李佳莹,付玮琦,任佳敏,李风娟
摘要(Abstract):
用解淀粉芽孢杆菌SY07(Bacillus amyloliquefaciens SY07)发酵绿豆,对发酵过程中绿豆的营养成分、体外降血糖活性和挥发性风味物质进行研究。结果表明,发酵24 h的绿豆具有最高的α-葡萄糖苷酶抑制活性和α-淀粉酶抑制活性,抑制率分别为50.3%和92.0%。发酵48 h的绿豆具有最高的总酚、总黄酮、多肽含量,分别为3.0 mg GAE/g DW,1.6 mg CE/g DW,5.4 g/100 g。在发酵过程中检测到了醇类、酮类、醛类、酸类、酯类、酚类、烷烃类、醚类、呋喃类、吡嗪类和其他类物质共96种挥发性风味成分,其中OAV>1的物质有11种。该研究表明B.amyloliquefaciens SY07发酵绿豆具有潜在良好的降血糖活性,可为开发新型发酵豆制品提供重要的参考。
关键词(KeyWords): 解淀粉芽孢杆菌SY07;营养成分;降血糖活性;风味成分
基金项目(Foundation): 天津市自然科学基金项目(16JCYBJC23200)
作者(Author): 赵雪如,李佳莹,付玮琦,任佳敏,李风娟
参考文献(References):
- [1]WOLDEMARIAMYOHANNES K,WAN Z,YU Q,et al.Prebiotic,probiotic,antimicrobial,and functional food applications of Bacillus amyloliquefaciens[J].Journal of Agricultural and Food Chemistry,2020,68(50):14709-14727.
- [2]刘润泽,王世伟,王卿惠,等.解淀粉芽孢杆菌酶系及其应用研究进展[J].高师理科学刊,2019,39(3):70-75.
- [3]YANG J,WU X B,CHEN H L,et al.A value-added approach to improve the nutritional quality of soybean meal byproduct:enhancing its antioxidant activity through fermentation by Bacillus amyloliquefaciens SWJS22[J].Food Chemistry,2019,272:396-403.
- [4]WU S,LU S,LIU J,et al.Physicochemical properties and bioactivities of rice beans fermented by Bacillus amyloliquefaciens[J].Engineering,2021,7(2):219-225.
- [5]LIANG Z,YI M,SUN J,et al.Physicochemical properties and volatile profile of mung bean flour fermented by Lacticaseibacillus casei and Lactococcus lactis[J].LWT-Food Science and Technology,2022,163:113565.
- [6]WU H,RUI X,LI W,et al.Mung bean (Vigna radiata) as probiotic food through fermentation with Lactobacillus plantarum B1-6[J].LWT-Food Science and Technology,2015,63(1):445-451.
- [7]王立,任思,周素梅,等.绿豆发酵制品及其功能活性研究[J].食品与机械,2019,35(2):231-236.
- [8]蔡尤林.一种具有溶栓功能的淡豆豉及其制备工艺研究[D].广州:华南农业大学,2016.
- [9]龙继艳,戴明,钟瑶,等.纳豆发酵原料筛选及其功能产品的开发[J].生物化工,2020(6):47-50.
- [10]YI C,LI Y,ZHU H,et al.Effect of Lactobacillus plantarum fermentation on the volatile flavors of mung beans[J].LWT-Food Science and Technology,2021,146:111434.
- [11]XIAO Y,WU X,YAO X,et al.Metabolite profiling,antioxidant and α-glucosidase inhibitory activities of buckwheat processed by solid-state fermentation with Eurotium cristatum YL-1[J].Food Research International,2021,143:110262.
- [12]ZHOU H,SAFDAR B,LI H,et al.Identification of a novel α-amylase inhibitory activity peptide from quinoa protein hydrolysate[J].Food Chemistry,2023,403:134434.
- [13]ROJAS-OCAMPO E,TORREJóN-VALQUI L,MU?óZ-ASTECKER L D,et al.Antioxidant capacity,total phenolic content and phenolic compounds of pulp and bagasse of four Peruvian berries[J].Heliyon,2021,7(8):7787.
- [14]JIA Z S,TANG M C,WU J M.The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals[J].Food Chemistry,1999,64(4):555-559.
- [15]WEIMER B C,OBERG C J,MOYES L V,et al.Comparison of classical ion exchange amino acid analysis and o-phthaldialdehyde methods to characterize proteolysis by Lactobacillus bulgaricus[J].Journal of Dairy Science,1989,72(11):2873-2876.
- [16]谢兰心,樊晓博.顶空固相微萃取法对两种大豆发酵豆豉挥发性成分的比较[J].中国调味品,2018,43(4):85-89.
- [17]LEE J H,HWANG C E,SON K S,et al.Comparisons of nutritional constituents in soybeans during solid state fermentation times and screening for their glucosidase enzymes and antioxidant properties[J].Food Chemistry,2019,272:362-371.
- [18]李风娟,刘鹏程,方元元,等.一株细菌发酵大豆的α-葡萄糖苷酶抑制活性研究[J].中国调味品,2016,41(8):26-30.
- [19]ZHENG Y,LIU S,XIE J,et al.Antioxidant,α-amylase and α-glucosidase inhibitory activities of bound polyphenols extracted from mung bean skin dietary fiber[J].LWT-Food Science and Technology,2020,132:109943.
- [20]戚平,曾羲,崔世博,等.扁竹根多肽的降糖活性研究[J].食品与发酵科技,2021,57(3):56-59,67.
- [21]JUAN M Y,CHOU C C.Enhancement of antioxidant activity,total phenolic and flavonoid content of black soybeans by solid state fermentation with Bacillus subtilis BCRC 14715[J].Food Microbiology,2010,27(5):586-591.
- [22]SUN N,XIE J Y,XIE J H,et al.Purification,composition and activity of bound polyphenols from mung bean coat dietary fiber[J].Food Research International,2022,162:111997.
- [23]SANJUKTA S,RAI A K.Production of bioactive peptides during soybean fermentation and their potential health benefits[J].Trends in Food Science & Technology,2016,50:1-10.
- [24]WEI G,REGENSTEIN J M,LIU X,et al.Comparative aroma and taste profiles of oil furu (soybean curd) fermented with different mucor strains[J].Journal of Food Science,2020,85(11):1642-1650.
- [25]段鑫锐,康波,张小龙,等.蚝油挥发性风味成分比较分析[J].中国调味品,2022,47(11):144-150.
- [26]张雅雯,钟源,郭爱玲,等.竹笋发酵中挥发性物质及菌群多样性动态分析[J].中国调味品,2023,48(1):1-7,13.