响应面法优化荖叶精油的提取工艺及精油化学成分的分析Optimization of Processing Technology of Volatile Oil from Leaves of Piper betle Linn by Response Surface Method and Analysis of Its Chemical Components
苏彦利,唐敏敏,陈卫军,王辉
摘要(Abstract):
利用同时蒸馏萃取技术,通过单因素实验优化,研究了物料粒径、料液比、超声时间、蒸馏时间、加盐量5个萃取条件对荖叶精油提取率的影响。在此基础上,使用响应面软件Design-Expert,选用BoxBehnken法(BBD)的四水平三因素对荖叶精油试验因素进行最佳优化,并采用气相色谱-质谱法(GCMS)对提取出的精油进行了成分分析。实验结果表明:荖叶精油的最佳提取条件为:破碎粒径64.50目,液料比8.29∶1,原料超声时间51.05 min,同时蒸馏萃取时间8.54h,响应面理论最优值21.87mg/g,实验验证平均值21.65mg/g。GC-MS对精油化学成分进行分析,共检测出41种物质,其中异丁香酚含量70.03%,对烯丙基苯酚含量10.52%,乙酰基丁香酚含量7.04%。
关键词(KeyWords): 荖叶精油;响应面;同时蒸馏萃取;超声波;GC-MS
基金项目(Foundation): 中央级公益性科研院所基本科研业务费专项项目(1630052015040)
作者(Author): 苏彦利,唐敏敏,陈卫军,王辉
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