吴滢滢,汪璇,陈晖,郑平,石昕芸,王伟平

• 1:湖北工业大学生命科学与健康工程学院
• 2:安琪酵母股份有限公司

摘要(Abstract)：

在固态发酵过程中添加ZnSO_4,发现ZnSO_4的添加导致红曲霉生物量明显降低，抑制烟灰色红曲霉(Monascus fuliginosus)AQB2的生长，有利于次级代谢产物Monacolin K的产生。当添加0.1%ZnSO_4时，MK产量最高，达到29.56 mg/g,较未添加ZnSO_4提高了2.18倍。进一步研究发现ZnSO_4的添加有利于形成酸式MK,且pH会影响酸式MK产生，pH升高，酸式MK产量也增加。当添加0.1%ZnSO_4时，酸式MK产量为23.98 mg/g,是未添加ZnSO_4时酸式MK产量的1.94倍，MK开环率为81%。ZnSO_4在提高MK总产量的同时也保持了较高的开环率，为工业化红曲霉固态发酵调控高产酸式MK提供了参考。

关键词(KeyWords)： 烟灰色红曲霉;Monacolin K;固态发酵;生物量;ZnSO_4

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31101349)

作者(Author): 吴滢滢,汪璇,陈晖,郑平,石昕芸,王伟平

参考文献(References)：

• [1]邢宏博，曾李，许赣荣，等.红曲菌主要的活性物质及红曲产品在饲料行业应用前景[J].广东饲料，2020(3):28-31.
• [2]徐晓飞，何瑞琪，宗敏华，等.红曲霉代谢产物的调控及在酿酒中的应用研究进展[J].中国酿造，2023(4):8-13.
• [3]王芳慧，张静，王昌禄，等.基于响应面的红曲色素液态发酵培养基优化[J].中国调味品，2021,46(12):1-5,26.
• [4]杜琳琳，赵祥颖，刘丽萍，等.红曲霉菌的筛选及其在液态食醋酿造中的应用[J].中国调味品，2024,49(1):33-39.
• [5]刘润璞，孙晓东，李雨恒，等.紫红曲霉A13、Fen发酵红曲米中莫纳克林K的含量测定[J].中国调味品，2020,45(8):132-134.
• [6]KRAIRAK S,YAMAMURA K,IRIE R,et al.Maximizing yellow pigment production in fed-batch culture of Monascus sp.[J].Journal of Bioscience and Bioengineering,2000,90(4):363-367.
• [7]ENDO A.Monacolin K,a new hypocholesterolemic agent that specifically inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase[J].The Journal of Antibiotics,1980,33(3):334-336.
• [8]ALBERTS A W,CHEN J,KURON G,et al.Mevinolin:a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent[J].Proceedings of the National Academy of Sciences,1980,77(7):3957-3961.
• [9]ANAGNOSTIS P,PASCHOU S A,GOULIS D G,et al.Dietary management of dyslipidaemias.Is there any evidence for cardiovascular benefit?[J].Maturitas,2018,108:45-52.
• [10]PATEL S.Functional food red yeast rice (RYR) for metabolic syndrome amelioration:a review on pros and cons[J].World Journal of Microbiology and Biotechnology,2016,32(5):87.
• [11]AGARWAL B,HALMOS B,FEOKTISTOV A S,et al.Mechanism of lovastatin-induced apoptosis in intestinal epithelial cells[J].Carcinogenesis,2002,23(3):521-528.
• [12]STUBBS R J,SCHWARTZ M,BAYNE W F.Determination of mevinolin and mevinolinic acid in plasma and bile by reversed-phase high-performance liquid chromatography[J].Journal of Chromatography B:Biomedical Sciences and Applications,1986,383:438-443.
• [13]TANG B K,KALOW W.Variable activation of lovastatin by hydrolytic enzymes in human plasma and liver[J].European Journal of Clinical Pharmacology,1995,47(5):449-451.
• [14]周香珍，林书发，何书华.红曲药学评价研究进展[J].中国现代中药，2016,18(7):936-941.
• [15]CHEN B J,HUANG X D,PENG H T,et al.Effectiveness and safety of red yeast rice predominated by Monacolin K β-hydroxy acid form for hyperlipidemia treatment and management[J].Journal of Traditional Chinese Medicine,2022,42(2):264-271.
• [16]齐文武，陈晓云，郑世茂，等.高产洛伐他汀的功能性红曲固态发酵营养液最佳pH值的研究[J].中国食物与营养，2017(4):34-37.
• [17]ZHANG J,WANG Y L,LU L P,et al.Enhanced production of Monacolin K by addition of precursors and surfactants in submerged fermentation of Monascus purpureus 9901[J].Biotechnology and Applied Biochemistry,2014,61(2):202-207.
• [18]ZHANG C,CHEN M X,ZANG Y M,et al.Effect of arginine supplementation on Monacolin K yield of Monascus purpureus[J].Journal of Food Composition and Analysis,2022,106:104252.
• [19]LIN L,WANG C L,LI Z,et al.Effect of temperature-shift and temperature-constant cultivation on the Monacolin K biosynthetic gene cluster expression in Monascus sp.[J].Food Technology and Biotechnology,2017,55(1):40-47.
• [20]黄颖颖，杨成龙，陈源.液态培养红曲霉产洛伐他汀开闭环组分的研究[J].福建农业学报，2013,28(8):817-822.
• [21]邱源，周立平，嘉晓勤.红色红曲菌液态发酵产Monacolin K重要影响因素的研究[J].酿酒科技，2010(5):47-50.
• [22]黄颖颖，陆东和，杨成龙，等.微量营养源对红曲霉产洛伐他汀开闭环组分的影响[J].福建农业学报，2015,30(3):286-292.
• [23]王玲，吴军林，吴清平.红曲降血脂功能的研究及应用概况[J].食品工业科技，2014,35(8):387-389,393.
• [24]CHAI X Y,AI Z L,LIU J,et al.Effects of pigment and citrinin biosynthesis on the metabolism and morphology of Monascus purpureus in submerged fermentation[J].Food Science and Biotechnology,2020,29(7):927-937.
• [25]邓光武，夏帆，王洁雅，等.低频交变磁场对红曲霉固态发酵生物量的影响[J].农业机械学报，2012,43(6):128-132.
• [26]杨润蕾，张美如，高雪慧，等.一株枯草芽孢杆菌进行饲料固态发酵的条件优化[J].工业微生物，2023,53(5):116-118.
• [27]BAU Y S,WONG H C.Zinc effects on growth,pigmentation and antibacterial activity of Monascus purpureus[J].Physiologia Plantarum,1979,46(1):63-67.
• [28]LI J,FENG L D,LI D,et al.ROS regulate NCF2,key metabolic enzymes and MDA levels to affect the growth of Fusarium solani[J].Agriculture,2022,12(11):1840.
• [29]陈慎，黄颖颖，陆东和，等.外加营养源对红曲霉固态发酵产莫纳可林K和洛伐他汀的影响[J].中国食品添加剂，2017(9):102-113.
• [30]WANG T H,LIN T F.Monascus rice products[J].Advances in Food and Nutrition Research,2007,53:123-159.
• [31]LEE C L,HUNG H K,WANG J J,et al.Improving the ratio of Monacolin K to citrinin production of Monascus purpureus NTU 568 under Dioscorea medium through the mediation of pH value and ethanol addition[J].Journal of Agricultural and Food Chemistry,2007,55(16):6493-6502.
• [32]文镜，顾晓玲，常平，等.紫外分光光度法测定红曲中酸式Lovastatin的含量[J].中国食品添加剂，2002(1):69-74.