赤小豆多糖提取工艺、改性及其特性研究Study on Extraction Technology, Modification and Characteristics of Polysaccharides from Vigna umbellata
裴肖茜,张蕴哲,李子坤,徐慧,李东晓,张伟
摘要(Abstract):
该研究以赤小豆为原料,采用超声辅酶法和水提醇沉工艺提取赤小豆胞内多糖,优化提取工艺,并通过超声波对其进行改性。研究改性前后赤小豆胞内多糖的抗氧化特性以及对果蔬的冷藏保鲜作用,以拓展其应用领域。研究结果表明,赤小豆多糖提取的最佳工艺条件为超声时间50 min、水提温度70℃、水提时间200 min、复合酶处理温度47℃,在此条件下,多糖提取率可达4.33%。赤小豆多糖体外抗氧化试验结果表明,改性赤小豆多糖对·OH、DPPH·、ABTS~+·的清除率分别为88.35%、83.67%、58.82%,比改性前分别提高了18.1%、17.4%、14.87%。多糖改性前后对草莓的冷藏保鲜试验结果显示,其保鲜能力排序为3%改性赤小豆多糖组>1%改性赤小豆多糖组>3%赤小豆多糖组>1%赤小豆多糖组。因此,该研究采用的提取工艺对赤小豆多糖的提取率较高,改性后的多糖对自由基的清除率明显高于改性前。改性前后的赤小豆多糖对草莓均具有保鲜效果,而改性后赤小豆多糖的保鲜效果优于改性前。
关键词(KeyWords): 多糖;赤小豆;提取工艺;抗氧化活性;果蔬保鲜
基金项目(Foundation): 国家自然科学基金项目(32172288,31371772);; 河北省自然科学基金重点项目(C2019204342);河北省自然科学基金项目(C2019204284);; 河北省杂粮杂豆产业技术创新团队质量提升与品牌培育专项(HBCT2023050204);; 中央引导地方科技发展资金项目(216Z5501G,226Z5503G,236Z5502G);; 河北省外专百人计划(360-0803-JSN-3YGS);; 河北农业大学食品加工学科群经费资助(2021-06);; 河北省重点研究开发计划项目(18275501D);; 河北省教育厅科研项目(QN2022073)
作者(Author): 裴肖茜,张蕴哲,李子坤,徐慧,李东晓,张伟
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