董科,陈宇航,沈丹芸,裴晓方

• 1:四川大学华西公共卫生学院(华西第四医院)
• 2:食品安全监测与风险评估四川省重点实验室

摘要(Abstract)：

采用平板法初筛,从市售水豆豉分离细菌中筛选得到6株具有高产蛋白酶、淀粉酶和豆豉纤溶酶的菌株;经化学法复筛,获得1株高产豆豉纤溶酶菌株B61。对B61菌株进行形态学、生理生化特性鉴定及16SrDNA同源性分析,最后鉴定为贝莱斯芽孢杆菌(Bacillus velezensis),且该菌株在生长16h后检测到最高豆豉纤溶酶活力(826.25IU/mL)。该研究丰富了豆豉纤溶酶产生菌的菌种资源,并为其后续相关研究提供了理论依据。

关键词(KeyWords)： 水豆豉;豆豉纤溶酶;筛选;菌种鉴定

Abstract:

Keywords:

基金项目(Foundation): 四川省科技厅科技支撑项目(2016SZ0034)

作者(Author): 董科,陈宇航,沈丹芸,裴晓方

参考文献(References)：

• [1]龚福明,柳陈坚,李海燕.豆豉纤溶酶的研究现状[J].生物技术通报,2009(11):34-38.
• [2]王成涛,郑杰,籍保平,等.豆豉纤溶酶Subtilisin FS33的溶栓作用及其机制的研究[J].营养学报,2007,29(6):600-604.
• [3]张雪,袁洪水,辛欣,等.豆豉纤溶酶组分S-7FE-1的酶学性质及体外溶栓特性分析[J].中国食品学报,2012,12(10):40-46.
• [4]Wang S L,Wu Y Y,Liang T W.Purification and biochemical characterization of a nattokinase by conversion of shrimp shell with Bacillus subtilis TKU007[J].N Biotechnol,2011,28(2):196-202.
• [5]Sumi H,Hamada H,Tsushima H,et al.A novel fibrinolytic enzyme(nattokinase)in the vegetable cheese natto;a typical and popular soybean food in the Japanese diet[J].Experientia,1987,43(10):1110-1111.
• [6]Chen H,Mcgowan E M,Ren N,et al.Nattokinase:apromising alternative in prevention and treatment of cardiovascular diseases[J].Biomark Insights,2018,13:918-920.
• [7]Kim W,Choi K,Kim Y,et al.Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp.strain CK 11-4 screened from Chungkook-Jang[J].Applied&Environmental Microbiology,1996,62(7):2482.
• [8]李江伟,冉国侠,陈新梅.豆豉溶栓酶的分离纯化及其体外溶栓作用[J].中国生化药物杂志,1999(3):148-150.
• [9]沈畅萱,王修俊,黄珊.豆豉纤溶酶等微生物源纤溶酶的研究与应用进展[J].中国酿造,2017,36(8):6-10.
• [10]GB 1886.174-2016,食品添加剂食品工业用酶制剂[S].
• [11]张旭,唐非,彭良斌,等.豆豉中解淀粉芽孢杆菌发酵产纤溶酶[J].食品研究与开发,2013,34(24):239-243.
• [12]孙园,牟光庆.豆豉纤溶酶产生菌的筛选及鉴定[J].食品研究与开发,2007(1):36-39.
• [13]曹凤明,杨小红,马鸣超,等.枯草芽孢杆菌近缘种群鉴定方法研究进展[J].微生物学通报,2014(5):968-974.
• [14]Dunlap C A,Kim S J,Kwon S W,et al.Bacillus velezensis is not a later heterotypic synonym of Bacillus amyloliquefaciens;Bacillus methylotrophicus,Bacillus amyloliquefaciens subsp.plantarum and Bacillus oryzicola are later heterotypic synonyms of Bacillus velezensis based on phylogenomics[J].Int J Syst Evol Microbiol,2016,66(3):1212-1217.
• [15]Ruiz-Garcia C,Bejar V,Martinez-Checa F,et al.Bacillus velezensis sp.nov.,a surfactant-producing bacterium isolated from the river Velez in Malaga,southern Spain[J].Int J Syst Evol Microbiol,2005,55(1):191-195.
• [16]Wang L T,Lee F L,Tai C J,et al.Bacillus velezensis is a later heterotypic synonym of Bacillus amyloliquefaciens[J].Int J Syst Evol Microbiol,2008,58(3):671-675.
• [17]Fan B,Blom J,Klenk H P,et al.Bacillus amyloliquefaciens,Bacillus velezensis,and Bacillus siamensis form an"Operational Group B.amyloliquefaciens"within the B.subtilis species complex[J].Frontiers in Microbiology,2017(8):22.
• [18]Hee C B,Hyun K K,Eun J S,et al.Genomic and metabolic features of the Bacillus amyloliquefaciens group—B.amyloliquefaciens,B.velezensis,and B.siamensis-revealed by pan-genome analysis[J].Food Microbiology,2004(3):207-220.
• [19]Yao Z,Kim J A,Kim J H.Characterization of a fibrinolytic enzyme secreted by Bacillus velezensis BS2isolated from sea squirt jeotgal[J].J Microbiol Biotechnol,2019(8):102-106.
• [20]吴拥军,贾东旭,王嘉福,等.豆豉芽孢杆菌前发酵条件初探与酶活力测定[J].食品工业科技,2010,31(9):191-194.