酵母味素综合抽提技术的研究Investigation on complex extraction technique of yeast gourmet
焦宇知
摘要(Abstract):
研究了从啤酒废酵母中抽提酵母味素的综合技术。结果表明,酵母味素的最佳工艺条件为:脱苦用NaHCO3为0.5%,采用30MPa压力两次均质8%~12%的酵母泥悬浮液,自溶初加入葡聚糖酶,自溶时加入3%的NaCl溶液,温度为55℃,自溶时间为24h,其中,自溶10h后加入2.0%的复合蛋白酶,自溶初始pH为7.0。自溶结束后加核苷酸酶水解3h,氨基氮含量为5.7g/L,氨基态氮得率为6.87%,核苷酸含量达到2.62%。产品口味纯正,香气浓郁。
关键词(KeyWords): 酵母味素;抽提;核苷酸
基金项目(Foundation): 江苏省“青蓝工程”资助
作者(Author): 焦宇知
参考文献(References):
- [1]Gaudreau H,Conway J,Champagne C P.productionof zinc-enriched yeast extracts[J].FoodSci Technol,2001,(4):348-351.
- [2]Lidia Koleva,Lubomira Stateva,Pencho Venkow.Non-wasteful fractionation of fragile yeast cells for theproduction of nutritional protein and other by-products[J].Z Lebensm Unters Forsch A,l999,208:439-443.
- [3]Hough.Js Yeast autolysis[J].Process Biochemstry,1970(5):50-52.
- [4]Verdnyn C.Effect of high pressure homogenization andpapain on the preparation of autolysed yeast extract[J].Enzyme Microb.Technol,1990,12(10):764-770.
- [5]Young CL.Development of a new processing methodand quality evaluation of yeast autolyzate[J].Korean Journal of Food Science and Technology,1993,25(1):78-82.
- [6]Prentice.N Characterization of a nuclease from maltedbarleyroots[J].Journal of Cereal Science,1 9 8 7(5):175-187.