筛选出芽短梗霉生产低聚果糖探究溶氧的影响Screening Aureobasidium pullulans for Producing Fructo-Oligofructoses and Exploring the Effect of Dissolved Oxygen
李成林,曹伟锋,秦林莉,刘郅骞,张静,刘思伽,竹源,梁欣泉
摘要(Abstract):
目的:从土壤树叶中筛选出芽短梗霉菌和实验室已有的出芽短梗霉,探究出芽短梗霉菌产低聚果糖的发酵性能。方法:对筛选的出芽短梗霉菌和实验室保存的出芽短梗霉菌进行发酵,通过高效液相色谱法测定得出最佳菌株,改变溶氧发酵条件探究对发酵产低聚果糖的影响。结果:通过筛选和发酵,筛选出产低聚果糖最高的出芽短梗霉菌IPE-3。结论:IPE-3是一种优秀的产低聚果糖的菌株,在600 r/min、1.8 L/min通气量和0 bar条件下,低聚果糖的产量高。
关键词(KeyWords): 出芽短梗霉菌;低聚果糖;筛选;优化
基金项目(Foundation): 中国科学院科技服务网络计划(KFJ-STS-QYZX-096)
作者(Author): 李成林,曹伟锋,秦林莉,刘郅骞,张静,刘思伽,竹源,梁欣泉
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