广式白腐乳风味特性的差异分析Difference Analysis of Flavor Characteristics of Cantonese White Fermented Bean Curd
江津津,董蕾,万红霞,张越华,钟研威,蔡施平
摘要(Abstract):
为探究广式白腐乳的风味特性差异,并探索咸鲜调味料的风味检测方法,采用气相色谱-离子迁移谱法(gas chromatography-ion mobility spectrometry, GC-IMS)、电子鼻、电子舌和氨基酸分析技术对5类广式白腐乳进行气味差异分析和滋味特性对比分析。结果表明,乙醇、乙酸、乙酸乙酯、丁酸丁酯、丁二酮、甲基丁醛、庚烯醛和丙酸等化合物是不同广式白腐乳中共有的高含量挥发性化合物。GHFR和LFFR与其他样品的气味差异明显。广式白腐乳的关键挥发性化合物包括乙醇、呋喃类、吡嗪类、二甲基三硫、糠醛、含氮化合物等。GHFR被鉴定出更多特征气味化合物。PCA分析结果表明,CBFR、HTFR和GZHFR的气味响应区域集中甚至重合,挥发性气味相近。电子舌分析结果表明,不同类样品在甜味、苦味、涩味和回味上的差异很小。游离氨基酸分析结果表明,LFFR总游离氨基酸含量最高,广式白腐乳均含有丰富的必需氨基酸和鲜味氨基酸。电子鼻、电子舌和GC-IMS等智能感官分析技术对广式白腐乳的风味甄别十分有效。5类广式白腐乳的气味差异明显,滋味差异不明显。GHFR和LFFR的风味更丰满,典型性突出。风味差异的主要原因是后期关键工序“灌汤后酵”时添加的酒和卤汁不尽相同。
关键词(KeyWords): 广式白腐乳;电子鼻;电子舌;气相离子迁移谱;风味差异
基金项目(Foundation): 2021年广东大学生科技创新培育专项资金项目(pdjh2022b0961);; 广州市2021年科技计划项目(202102080487);; 广州城市职业学院科研团队“新资源食品功能与风味研究开发团队”;广州城市职业学院大学生创新创业训练计划项目(DC2022005);; 中国高校产学研创新基金-新一代信息技术创新项目立项课题(2020ITA06007);; 广州市大学生创新创业训练计划项目(2022CXCYJH013);; 广州市名师工作室项目(2022MSGZS015)
作者(Author): 江津津,董蕾,万红霞,张越华,钟研威,蔡施平
参考文献(References):
- [1]周小虎,李理.白腐乳发酵过程中细菌群落演替规律研究[J].中国酿造,2020,39(5):168-172.
- [2]XIE C,ZENG H,WANG C,et al.Volatile flavour components,microbiota and their correlations in different sufu,a Chinese fermented soybean food[J].Journal of Applied Microbiology,2018,125(6):1761-1773.
- [3]XU D D,WANG P,ZHANG X,et al.High-throughput sequencing approach to characterize dynamic changes of the fungal and bacterial communities during the production of sufu,a traditional Chinese fermented soybean food[J].Food Microbiology,2020,86:103340.
- [4]周子文.白腐乳工艺优化及品质分析研究[D].广州:仲恺农业工程学院,2020.
- [5]YANG J,SUN W D,CUI C,et al.Gamma-glutamylation of the white particulates of sufu and simultaneous synthesis of multiple acceptor amino acids-containing gamma-glutamyl peptides:favorable catalytic actions of glutaminase[J].LWT-Food Science and Technology,2018,96:315-321.
- [6]FEI Y,LI L,CHEN L,et al.High-throughput sequencing and culture based approaches to analyze microbial diversity associated with chemical changes in naturally fermented tofu whey,a traditional Chinese tofu-coagulant[J].Food Microbiology,2018,76:69-77.
- [7]蒋丽婷,李理.不同品牌白腐乳风味成分的比较[J].食品工业科技,2012,33(8):99-120.
- [8]LIU C,WANG X S,MA H,et al.Functional properties of protein isolates from soybeans stored under various conditions[J].Food Chemistry,2008,111:29-37.
- [9]CHUNG H Y.Volatile components in fermented soybean (Glycine max) curds[J].Journal of Agricultural and Food Chemistry,1999,47(7):2690-2696.
- [10]HAN B Z,ROMBOUT F M,NOUT M J R,et al.Chinese fermented soybean food[J].International Journal of Food Microbiology,2001,6:1-10.
- [11]HWAN C H,CHOU C C.Volatile components of the Chinese fermented soya bean curd as affected by the addition of ethanol in aging solution [J].Journal of the Science of Food and Agriculture,1999,43:2449-2452.
- [12]YANG J,DING X,QIN Y,et al.Safety assessment of the biogenic amines in fermented soya beans and fermented bean curd[J].Journal of Agricultural and Food Chemistry,2014,62(31):7947-7954.
- [13]林丹,胡金祥,何蓓蓓,等.电子鼻和电子舌数据融合在鱼香调味汁风味识别中的应用[J].中国调味品,2021,46(9):145-150.
- [14]王建伟,叶升.电子鼻在食品行业的应用进展[J].中国调味品,2022,47(10):198-200,205.
- [15]QIAN C,HU Y Y,WEN R X,et al.Characterisation of the flavour profile of dry fermented sausages with different NaCl substitutes using HS-SPME-GC-MS combined with electronic nose and electronic tongue[J].Meat Science,2020,172:108338.
- [16]DONG W J,HU R S,LONG Y Z,et al.Comparative evaluation of the volatile profiles and taste properties of roasted coffee beans as affected by drying method and detected by electronic nose,electronic tongue,and HS-SPME-GC-MS[J].Food Chemistry,2019,272:723-731.
- [17]REBECCA N B,HERBERT S,TSUNG T.Comparison of sensory and consumer results with electronic nose and tongue sensors for apple juices[J].Food Quality and Preference,2002,13(6):409-422.
- [18]ZHANG L,HU Y Y,WANG Y,et al.Evaluation of the flavour properties of cooked chicken drumsticks as affected by sugar smoking times using an electronic nose,electronic tongue,and HS-SPME/GC-MS[J].LWT-Food Science and Technology,2021,140:110764.
- [19]WANG D,LU R R,MA Y,et al.Development of volatile compounds fingerprints by headspace gas chromatography-ion mobility spectrometry in concentrated tomato paste and distillate during evaporation processing[J].International Journal of Food Science and Technology,2020,56(6):14901.
- [20]叶丹,王传明,刘鹏,等.分子感官科学技术在调味品上的应用研究进展[J].中国调味品,2021,45(5):198-200.
- [21]叶洵,刘子博,张婷,等.基于GC-MS结合保留指数法建立花椒挥发油指纹图谱[J].中国调味品,2022,47(4):68-73.
- [22]袁小钧,钟世荣,吴华昌,等.火锅常用不同品种干辣椒感官品质差异研究[J].中国调味品,2022,47(4):173-177.
- [23]郑小嘎,吴爱东,陈庆彩,等.不同产地大马士革玫瑰精油特征成分的气相离子迁移谱对比分析[J].食品安全质量检测学报,2020(11):3465-3472.
- [24]梁天一,杨娟,董浩,等.基于GC-IMS技术鉴别不同年份新会陈皮中的挥发性风味物质[J].中国调味品,2020,45(4):168-173.
- [25]王鹏,王文平,续丹丹,等.红腐乳发酵过程中滋味化合物分析及电子舌鉴别[J].食品科学2021,42(14):170-179.
- [26]郭云霞,李娜,程伟,等.液液萃取与顶空固相微萃取结合气相色谱-质谱法分析金种子馥合香白酒香气成分的比较[J].食品安全质量检测学报,2021,12(23):9056-9061.
- [27]CAVANNA D,ZANARDI S,DALLASTA C,et al.Ion mobility spectrometry coupled to gas chromatography:a rapid tool toassess eggs freshness[J].Food Chemistry,2019,271:691-696.
- [28]CHEN T,LIU C Y,MENG L L,et al.Early warning of rice mildew based on gas chromatography-ion mobility spectrometry technology and chemometrics[J].Journal of Food Measurement and Characterization,2021,15(2):1939-1948.
- [29]ALIA?O-GONZáLEZ M J,FERREIRO-GONZáLEZ M,ESPADA-BELLIDO E,et al.Novel method based on ion mobility spectroscopy for the quantification of adulterants in honeys[J].Food Control,2020:114:107236.
- [30]LI W,CHEN Y P,BLANK I,et al.GC×GC-ToF-MS and GC-IMS based volatile profile characterization of the Chinese dry-cured hams from different regions[J].Food Research International,2021,142(7-8):110222.
- [31]江津津,欧爱芬,潘光健,等.不同产地传统海虾酱的风味特征[J].水产学报,2021,45(12):2072-2082.
- [32]丁素君.莫尔法测定酱油和食醋中的氯化钠含量[J].实验室科学,2016,19(3):21-22,25.