高良姜水提物的体外抗氧化和胰脂肪酶、α-葡萄糖苷酶抑制功能研究Antioxidant Activities and Inhibitory Effects Against Pancreatic Lipase and α-Glucosidase of Galangal Aqueous Extracts in Vitro
陈浩南,李姣,王婉愉,张晓峰,樊剑鸣
摘要(Abstract):
以新鲜高良姜为原料,经冻干脱脂处理制备水提物,测定高良姜水提物中多酚和黄酮含量,使用ABTS法、DPPH法和FRAP法评价水提物的抗氧化能力,同时测定其对α-葡萄糖苷酶和胰脂肪酶的抑制功能。结果显示:高良姜水提物中多酚和总黄酮分别为45.73mg GAE/g DW和0.41mg QE/g DW。水提物对ABTS和DPPH自由基离子的半抑制浓度IC50分别为2.55,8.78 mg/mL。FRAP值为382.14μmol Fe2+/g DW。高良姜水提物对α-葡萄糖苷酶和胰脂肪酶抑制活性的IC50值分别为14.45,543.51mg/mL。表明高良姜水提物中含有丰富的多酚类化合物,具有较强的体外抗氧化能力和α-葡萄糖苷酶和胰脂肪酶抑制功能。
关键词(KeyWords): 高良姜;多酚;总黄酮;抗氧化活性;酶抑制活性;α-葡萄糖苷酶;胰脂肪酶
基金项目(Foundation): 河南省科技攻关计划项目(152102310260)
作者(Author): 陈浩南,李姣,王婉愉,张晓峰,樊剑鸣
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