纤维素酶法提取百里香黄酮的工艺优化研究Study on Extraction Technology of Flavonoids from Thyme by Cellulase
王娣,柯春林,曹珂珂,谢海伟,李妍
摘要(Abstract):
利用纤维素酶对百里香中黄酮进行了提取工艺研究。考察了酶解温度、酶量、pH和酶解时间对黄酮得率的影响。百里香黄酮最佳提取工艺:酶解温度45℃,酶量0.2%,pH 5.0,酶解时间1.5h,黄酮得率可达1.528%。抗氧化实验表明百里香黄酮具有较强的抗氧化活性,黄酮提取物浓度为1.0mg/mL时,对DPPH自由基的清除率达73.84%,对羟基自由基的清除率为51.73%。
关键词(KeyWords): 纤维素酶;黄酮;正交试验;抗氧化
基金项目(Foundation): 安徽省教育厅重点科研项目(KJ2015A204)
作者(Author): 王娣,柯春林,曹珂珂,谢海伟,李妍
参考文献(References):
- [1]中国科学院植物研究所.中国高等植物图鉴(第三册)[M].北京:科学出版社,2002:681.
- [2]Bensmira M,Jiang B,Nsabimana C,et al.Effect of lavender and thyme incorporation in sunflower seed oil on its resistance to frying temperatures[J].Food Research International,2007,40(3):341-346.
- [3]Solmakos N,Govaris A,Koidis P,et al.The antimicrobial effect of thyme essential oil,nisin,and their combination against Listeria monocytogenes in minced beef during refrigerated storage[J].Food Microbiology,2008,25(1):120-127.
- [4]Iraj Rasooli,Mehdi Razzaghi Abyaneh.Inhibitory effects of thyme oils on growth and aflatoxin production by Aspergillus parasiticus[J].Food Control,2004,15:479-483.
- [5]Seung J L,Katumi U,Shibamoto T,et al.Identification of volatile components in basil(Ocimum basilicuml)and thyme leaves(Thymus vulgaris L.)and their antioxidant properties[J].Food Chemistry,2005(91):131-137.
- [6]Chu C L,Liu W T,Zhou T.Fumigation of sweet cherries with thymol and acid to reduce post harvest brown rot and blue mold rot[J].Fruits,2001(56):123-130.
- [7]Solimana K M,Badeaab R I.Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi[J].Food and Chemical Toxicology,2002(40):1669-1675.
- [8]Liu W T,Chu C L,Zhou T.Thymol and acetic acid vapors reduce post harvest brown rot of apricot and plums[J].Hort Science,2001(37):151-156.
- [9]刘智峰.酶法-超声波辅助提取香椿叶中总黄酮及抗氧化活性研究[J].食品工业科技,2015(20):314-319.
- [10]陈佳,徐怀德,米林峰,等.洋葱皮总黄酮纤维素酶法提取及抗氧化研究[J].食品科学,2011,32(4):37-41.
- [11]方芳,许凯扬,朱强,等.超声波辅助水酶法萃取葫芦籽油的研究[J].中国粮油学报,2012,27(10):62-66.
- [12]施英英,薛培俭,夏黎明.酶法提取葛根渣中异黄酮的研究[J].林产化学与工业,2006,26(1):62-64.
- [13]王岩岩,李文娟.纤维素酶提取陈皮黄酮的工艺条件[J].食品与生物技术学报,2008,27(2):71-74.
- [14]Paryathy K S,Negi P S,Srinivas P.Antioxidant,antimutagenic and antibacterial activities of curcumin-[betta]-diglucoside[J].Food Chemistry,2009,115:265-270.
- [15]Liu H,Qiu N,Ding H,et al.Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses[J].Food Research International,2008,41:363-365.