市售酱油中大肠杆菌失活模型的构建Establishment of Inactivation Models for Escherichia coli in Soy Sauce
章志超
摘要(Abstract)：
为有效防控大肠杆菌给酱油产品带来的质量隐患,通过将大肠杆菌接种于不同等级的生抽酱油和老抽酱油中,监测了不同酱油的氨基酸态氮、可溶性无盐固形物、pH、盐分、全氮和防腐剂添加量等理化指标,同时分析了不同数学模型对描述大肠杆菌在酱油中生长动态的适用性,并对预测模型的可靠性进行了评价。结果表明:不同酱油样品的质量等级均能达到国标要求,老抽酱油的可溶性无盐固形物和盐分均显著高于生抽酱油;酱油质量等级和贮藏温度是影响大肠杆菌失活速率的最主要因素,其中在特级老抽酱油中失活速率最小,而在三级酱油中失活最快,2~8℃冷藏能够显著延缓大肠杆菌的失活;Weibull模型对大肠杆菌在不同酱油中的生长动态的拟合优度R2=0.94~0.99,准确度Af为1.22~2.34,偏差度Bf均为1.00~1.02,预测效果最好;而Logistic模型和修正的Gompertz模型则在多数情况下预测误差较大,预测效果不理想。
关键词(KeyWords)： 大肠杆菌;酱油;失活模型
基金项目(Foundation):
作者(Author): 章志超
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