曲霉型豆豉曲中微生物分布The diversity of microorganisms in Aspergillus——type Douchiqu
张建华,李辉,李里特
摘要(Abstract):
以湖南浏阳一品香豆豉曲和天马山豆豉曲为对象,对曲中微生物分布进行研究。结果表明天马山和一品香豆豉曲中细菌总数、芽孢菌和霉菌分别为9.42 lg CFU/g,5.66 lg CFU/g,7.15 log CFU/g和8.93 lg CFU/g,5.65 lg CFU/g,7.11 log CFU/g,酵母和耐盐酵母较少,曲霉菌与总霉菌的比例分别为90.4%和95.1%。根据菌株在不同培养基上的茵落形态,及其菌丝形态、产孢结构和孢子形态等对曲霉进行了鉴定,结果表明主要曲霉菌为埃及曲霉、米曲霉原变种等。
关键词(KeyWords): 豆豉;曲霉;鉴定
基金项目(Foundation):
作者(Author): 张建华,李辉,李里特
参考文献(References):
- [1]I.Wiesel,H.J.Rehm,and B.Bisping,Improvement of tempeh fermentations by application of mixed cultures consisting of Rhizopus sp.And bacterial strains.Applied Microbiology and Biotechnology,1997,(47):218—225.
- [2]Sylvia Keuth and B.Bisping,Formation of vitamins by pure cultures of tempeh moulds and bacteria during the tempeh solid substrate fermentation [J].Journa of Applied Bacteriology,1993,(75):427—434.
- [3]B.S.Luh,Industrial production of soy sauce.Journal of Industrial Microbiology,1995,(14):467—471.
- [4]K.H.Steinkraus,1996,Indigenous fermented foods involving an alkaline fermentation.In:Handbook of Indigenous Fermented Foods (2 nd ed.),Steinkraus K H.Marcel Dekker,New York,USA.
- [5]Jian-Hua Zhang,Eizo Tatsumi,Chang—He Ding,Li—Te Li.Angiotensin I—Converting Enzyme Inhibitory Peptides in Douchi,a Chinese Traditional Fermented Soybean Product.Food chemistry,in press.
- [6]Fujita,H.,Yamagami,T.,andOhshima,K.(2001a).Efficacy and safety of touchi extract,anα—glucosidase inhibitor derived from fermentedsoybeans,in non—insulin—dependent diabetic mellitus.Journal of Nutrition Biochemistry,12:351—356.
- [7]Fujita,H.,Yamagami,T.,and Ohshima,K.(2001b).Long—term ingestion of a fermented soybean—derived touchi—extract withα—glucosidase inhibitory activity is safe and effective in humans with borderline and mild type—2 diabetes.Journal of Nutrition,131:2105—2108.
- [8]Hiroyuki Fujita,Tomohide Yamagami,Kazunori ohshima.Fermented soybean—drived water soluble touchi extract inhibitsα—glucosidase and is antiglycemicin rats and humans after single oral treatments.Journal of Nutrition,2001,(131):1211—1213.
- [9]9.Hiroyuki Fujita,Tomohide Yamagami.Fermented soy-bean-derived touchi—extract with anti—diabeticeffect viaα—glucosidase inhibitory action in along—term administration study with KKAy mice.Life Sciences,2001,(70):219—227.
- [10]诸葛健,王正祥.工业微生物实验技术手册[M],北京:中国轻工业出版社,1994.
- [11]魏景超,真菌鉴定手册[M],上海:上海科技出版社,1997.
- [12]Robert A.Samson,Ellen S.Hoekstra,Jens C.Frisvad,et al.Introduction to Food and Airbrone Fungi.2001.ASM Press.
- [13]齐祖同,中国真菌志[M],北京:科学出版社,1997.
- [14]Kathleen A.Hachmeister and Daniel Y.C.Fung,Tempeh:a mold—modified indigenous fermented food made from soybeans and/or cereal grains.Critical Reviews in Microbiology.1993(3):137—188.
- [15]R.A.Samson,J.A.VanKooij,andE.DE.Boer,Microbiological quality of commercial tempeh in The Netherlands.Journal of Food Protection,1987,50 (2):92—94.
- [16]Shibuya—I.,Tsuchiya—K.,Tamura—G.,et al.,Overproduction of an alpha—amylase/glucoamylase fusion protein in Aspergillus oryzae using a high expression vector.Bioscience,Biotechnology and Biochemistry,1992,56:(10):1674—1675.
- [17]Sriram—Padmanabhan,Murthy M.V.R.,Lonsane,B.K.,etal.Potential of Aspergillus oryzae CFTRI 1480 for producing proteinase in high titres by solid state fermentation.Applied Microbiology and Biotechnology,1993,(40):4,499—503.
- [18]Hirano,K.,Adachi,Y.,Ishibashi,S.,etal.,A survey for the aflatoxins in secondary metabolites produced by Aspergillus oryzae and Aspergillus sojae by enzyme—linked immunosorbent assay and high performance liquid chromatography.Proceed.Japanese Association of Mycotoxicology.1993,(37):37—42.
- [19]蔺新英,梅行,赵秀兰,等,大蒜抑制寄生曲霉生长及其产生黄曲霉毒素之初步研究[J],营养学报,1995,17(4):428—430.
- [20]刘江,孟昭赫.烟曲霉震颤素高产菌株的筛选[J],中国食品卫生杂志,1996,8(4):1—4.