董世荣,马春雪,贺泓睿

• 1:哈尔滨学院食品工程学院

摘要(Abstract)：

玉米醇溶蛋白分子刚性较强，利用酸诱导脱酰胺提高玉米醇溶蛋白的分子柔性。采用反溶剂法制备柔性玉米醇溶蛋白纳米颗粒，研究其对叶黄素的负载能力。结果表明，柔性玉米醇溶蛋白可以自组装形成纳米颗粒，柔性玉米醇溶蛋白纳米颗粒对叶黄素具有较好的运载能力。柔性玉米醇溶蛋白纳米颗粒表面或总叶黄素的含量均高于天然玉米醇溶蛋白纳米颗粒。叶黄素添加量为4.8%时，柔性玉米醇溶蛋白纳米颗粒表面和总叶黄素含量分别达到0.527μg/mL和1.411μg/mL。柔性玉米醇溶蛋白纳米颗粒对叶黄素的高负载能力归因于蛋白质分子的无序化，提高了与叶黄素的结合能力。玉米醇溶蛋白二级结构的改变源于脱酰胺改变了蛋白质的氨基酸组成。

关键词(KeyWords)： 玉米醇溶蛋白;脱酰胺反应;叶黄素;二级结构;氨基酸组成

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31801485);; 黑龙江省自然科学基金(LH2020C064);; 黑龙江省大学生创新创业项目(202110234043,202010234015)

作者(Author): 董世荣,马春雪,贺泓睿

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