预超声处理芦丁-大豆分离蛋白复合物对猪肌原纤维蛋白凝胶性及肉糜饼食用贮藏品质特性的影响Effects of Pre-Ultrasonic Treatment of Rutin-Soybean Protein Isolate Complex on Gel Properties of Porcine Myofibrillar Protein and Edible and Storage Quality Characteristics of Minced Meat Patties
赵钜阳,李玉奇,杜威,金美琳,刘明,王鹏宇
摘要(Abstract):
猪肉糜在食品加工、餐饮和家庭烹饪等领域应用广泛。然而,猪肉蛋白质中的关键组成成分——肌原纤维蛋白(myofibrillar protein, MP)容易氧化、热稳定性差,致使肉类产品腐败变质,货架期缩短,从而引起肉类品质下降。文章以芦丁-大豆分离蛋白(soybean protein isolate, SPI)复合物为研究对象,将不同超声处理下的芦丁-SPI复合物与MP复合成凝胶,用荧光光谱测定其结构的变化,制备不同超声预处理的芦丁-SPI-MP复合凝胶。对复合凝胶的硬度、持水性、溶解性等相关指标进行测定。最后将芦丁-SPI复合物应用在猪肉饼中,测定其感官品质、质构特性、水分含量和蒸煮损失率。结果显示,随着超声功率的增大,荧光强度明显减弱,芦丁-SPI复合物和MP的相互作用使芦丁-SPI-MP复合凝胶的硬度增强。熟肉饼具有更好的抗氧化和保鲜效果,而复合体明显提升了熟肉饼的感官品质,维持其滋润度。该研究有效解决了肉类产品易于氧化和发生腐败的难题,有望延长肉制品的货架期并为肉制品的品质改善提供理论依据。
关键词(KeyWords): 芦丁;大豆分离蛋白;超声;肌原纤维蛋白;凝胶性
基金项目(Foundation): 黑龙江省自然科学基金项目(LH2024C068);; 烹饪科学四川省高等学校重点实验室开放基金项目(PRKX2024Z13);; 广西高校桂菜工业化加工与安全重点实验室开放课题(2024KLGC0201);; 黑龙江省博士后基金面上项目(LBH-Z22204)
作者(Author): 赵钜阳,李玉奇,杜威,金美琳,刘明,王鹏宇
参考文献(References):
- [1]PAN N,BAI X,KONG B H,et al.The dynamic change in the degradation and in vitro digestive properties of porcine myofibrillar protein during freezing storage[J].International Journal of Biological Macromolecules,2023,234:123682.
- [2]??OJI?? B,MILO ??EVI?? S,SAVANOVI?? D,et al.Isolation,bioactive potential,and application of essential oils and terpenoidrich extracts as effective antioxidant and antimicrobial agents in meat and meat products[J].Molecules,2023,28(5):2293.
- [3]李颖畅,赵楠,李园园,等.儿茶素对鱿鱼肌肉-TMAOase体系中肌原纤维蛋白功能特性的影响[J].中国食品学报,2021,21(12):46-54.
- [4]王一郎,邹智芸,王以澄,等.不同种类植物多酚对冷藏牦牛肉糜肌原纤维蛋白氧化特性、功能特性及结构的影响[J].食品与发酵工业,2024,50(17):246-254.
- [5]贾娜,林世文,刘丹,等.芦丁诱导猪肉肌原纤维蛋白结构变化对蛋白凝胶特性的改善作用[J].食品科学,2021,42(8):67-73.
- [6]邵晓,张宁,孙乐彤,等.槲皮素和芦丁对猪肉肌原纤维蛋白结构的影响[J].食品研究与开发,2019,40(2):26-30.
- [7]DENG X X,CHEN Z,HUANG Q,et al.Spray-drying microencapsulation ofβ-carotene by soy protein isolate and/or OSA-modified starch[J].Journal of Applied Polymer Science,2014,131(12):40399.
- [8]NOSHAD M,MOHEBBI M,KOOCHEKI A,et al.Microencapsulation of vanillin by spray drying using soy protein isolate-maltodextrin as wall material[J].Flavour and Fragrance Journal,2015,30(5):387-391.
- [9]ZHAO J Y,CHEN Y Y,XU S,et al.High internal phase emulsion stabilized by soy protein isolate-rutin complex:rheological properties,bioaccessibility and in vitro release kinetics[J].International Journal of Biological Macromolecules,2024,280:135748.
- [10]WU Q Z,LYU D D,MAO X Y.Polyphenol removal with ultrasound-assisted ethanol extraction from defatted walnut powder:optimization of conditions and effect on functional properties of protein isolates[J].Journal of the Science of Food and Agriculture,2023,103(14):6995-7005.
- [11]LIU J Y,SONG G S,ZHOU L K,et al.Recent advances in the effect of ultrasound on the binding of proteinpolyphenol complexes in foodstuff[J].Food Frontiers,2023,4(2):721-732.
- [12]袁惠萍,顾丽雅,赵钜阳,等.芦丁-大豆分离蛋白输送载体片剂的制备工艺及其理化性质研究[J].粮食与油脂,2024,37(5):125-131.
- [13]杜汶清,张佳敏,刘琰艳,等.低温真空长时煮制时间对猪肉肌原纤维蛋白结构和特性的影响[J].中国调味品,2025,50(1):78-84.
- [14]SINNHUBE R O,YU T C.The 2-thiobarbituric acid reaction,an objective measure of the oxidative deterioration occurring in fats and oils[J].Japan Oil Chemists'Society,1977,26(5):259-267.
- [15]LI T,WANG L,CHEN Z X,et al.Functional properties and structural changes of rice proteins with anthocyanins complexation[J].Food Chemistry,2020,331:127336.
- [16]BUAMARD N,ENJKULS.Improvement of gel properties of sardine(Sardinella albella)surimi using coconut husk extracts[J].Food Hydrocolloids,2015,51:146-155.
- [17]王可.木质化鸡胸肉分级模型构建与过氧化氢体外氧化肌原纤维蛋白劣变机制及功能特性改善[D].泰安:山东农业大学,2023.
- [18]赵钜阳,袁惠萍,孙昕萌,等.槲皮素、芦丁与大豆分离蛋白非共价作用机制及其功能性和消化性研究[J].食品工业科技,2022,43(15):73-80.
- [19]VARELTZIS P,HULTIN H O,AUTIO W R.Hemoglobinmediated lipid oxidation of protein isolates obtained from cod and haddock white muscle as affected by citric acid,calcium chloride and pH[J].Food Chemistry,2008,108(1):64-74.
- [20]小路.欧盟关于“水产品挥发性盐基总氮(TVB-N)的限量标准及其测定方法”[J].科学养鱼,2002(12):58.
- [21]杨蓉.大豆分离蛋白/支链淀粉调控EGCG-蛋白互作对肌原纤维蛋白凝胶特性的影响[D].咸阳:西北农林科技大学,2022.
- [22]李湘銮,刘巧瑜,白卫东,等.食品添加剂对反复冻融猪肉蒸煮损失率的影响[J].中国调味品,2020,45(6):98-102.
- [23]XIN X J,QIU W,XUE H,et al.Improving the gel properties of salted egg white/cooked soybean protein isolate composite gels by ultrasound treatment:study on the gelling properties and structure[J].Ultrasonics Sonochemistry,2023,97:106442.
- [24]WEN P P,XIA C,ZHANG L,et al.Effects of different dry heating temperatures on the spatial structure and amino acid residue side-chain oxidative modification of soybean isolated proteins[J].Food Chemistry,2023,405:134795.
- [25]YAO W J,HUANG X N,LI C,et al.Underlying the effect of soybean oil concentration on the gelling properties of myofibrillar protein-based emulsion gels:perspective on interfacial adsorption,rheological properties and protein conformation[J].Food Hydrocolloids,2025,162:110935.
- [26]WEI S M,LI X,ZHANG J M,et al.Effects of cross-linked/acetylated tapioca starches on the gelling properties,rheological behaviors and microstructure of myofibrillar protein gels:perspective on molecular interactions and phase transition[J].Meat Science,2025,220:109703.
- [27]简华君.大豆分离蛋白对肌原纤维蛋白凝胶性质的影响[D].无锡:江南大学,2010.
- [28]陈金玉,胡方洋,吉芷芮,等.豌豆抗性淀粉对肌原纤维蛋白乳液凝胶特性及蛋白质体外消化率的影响[J].食品科学,2024,45(24):26-35.
- [29]石长波,姚恒喆,袁惠萍,等.儿茶素/大豆分离蛋白复合物对猪肉肠品质的影响[J].中国调味品2021,46(5):60-65.
- [30]张鑫,王宇波,黄志清,等.猪肉品质营养调控的研究进展[J].动物营养学报,2020,32(10):4555-4564.
- [31]经琳,杜宇明,赵祎,等.动植物双蛋白肉糜凝胶特性[J].食品与发酵工业,2024,50(21):240-248.
- [32]蒙志明,刘莹,席越阳,等.藜麦蛋白对低钠盐体系中猪肉肌原纤维蛋白凝胶特性的影响[J].肉类研究,2023,37(5):31-38.
- [33]常海军,石源伟,伯朝英,等.氧化条件下姜黄素对猪肉肌原纤维蛋白理化和凝胶特性的影响[J].食品科学,2024,45(8):63-71.