纳豆枯草芽孢杆菌对老陈醋中阿魏酸的影响Effect of Bacillus subtilis natto on Ferulic Acid in Aged Vinegar
董红兵,蔡凯,黄程,龚元元,汪超,祁勇刚,张亮子
摘要(Abstract):
以纳豆枯草芽孢杆菌为研究对象,用于以玉米为原料的老陈醋发酵。结果表明,糖化酒精发酵第3天接种5.0%的纳豆枯草芽孢杆菌种子液,醋酸发酵结束时的醋汁中阿魏酸含量高达59.36 mg/L。纳豆枯草芽孢杆菌分别接种于玉米、麸皮和谷糠浆液,阿魏酸含量随着发酵时间的延长均先显著增大而后缓慢减小,均在24 h时达到最大值,分别为3.729,1.018,0.661 g/L,而谷糠不粉碎时,阿魏酸缓慢增加,至36 h时其含量为0.087 g/L,仅为粉碎谷糠发酵24 h时的13.16%。采用老陈醋传统酿造工艺,接种纳豆枯草芽孢杆菌酿造的老陈醋,阿魏酸含量为32.45 mg/L,是不接种老陈醋的3.7倍、高粱特级老陈醋的近10倍。添加纳豆枯草芽孢杆菌种子液,提高了老陈醋阿魏酸含量,为工业化生产提供了理论基础。
关键词(KeyWords): 纳豆枯草芽孢杆菌;老陈醋;阿魏酸;糖化;醋酸发酵
基金项目(Foundation): 国家自然科学基金项目(32001705);; 湖北省教育厅科学技术研究项目(B2017567)
作者(Author): 董红兵,蔡凯,黄程,龚元元,汪超,祁勇刚,张亮子
参考文献(References):
- [1]SOHN Y T,OH J H.Characterization of physicochemical properties of ferulic acid[J].Arch Pharm Res,2003,26(12):56-58.
- [2]王宏伟,石二霞.阿魏酸及其衍生物的药理学研究进展[J].中药材,2007,29(6):601-603.
- [3]赵文红,邓泽元,范亚苇,等.阿魏酸体外抗氧化作用研究[J].食品科学,2010,31(1):219-223.
- [4]KIM H,OH E,MUM J,et al.Oxidative damages in the DNA,lipids,and proteins of rats exposed to isofluranes and alcohols[J].Toxicology,2006,220(2):169-178.
- [5]ITAGAKI S,KUROKAWA T,NAKATA C,et al.In vitro and in vivo antioxidant properties of ferulic acid:a comparative study with other natural oxidation inhibitors[J].Food Chemistry,2009,114(25):466-471.
- [6]WARGOVICH M J,JIMENEZ A,MCKEE K,et al.Efficacy of potential chemo preventive agents on rat colon aberrant crypt formation and progression[J].Carcinogenesis,2000,21(6):1149-1155.
- [7]YUAN Xiaoping,WANG Jing,YAO Huiyuan.Antioxidant activity of feruloylated oligosaccharide from wheat bran[J].Food Chem,2005,90:759-764.
- [8]RONDINI L,PEYRAT-MAILLARD M,MARSSET-BAGLIERI A,et al.Bound ferulic acid from bran is more bioavailability than the free compound in rat[J].J Agri Food Chem,2004,52:4338-4343.
- [9]刘丽萍.玉米中阿魏酸分布的研究[J].粮油加工,2008(12):82-83.
- [10]欧仕益,张颖,张景,等.碱解麦麸制备阿魏酸的研究[J].食品科学,2002,23(8):162-165.
- [11]FAULDS C B,WILLIAMSON G.Release of ferulic from wheat bran by a ferulic acid esterase (FEA-Ⅲ) from Aspergillus niger[J].Appl Microbiol Biotechnol,1995,43:1082-1087.
- [12]HOA T T,MANH V D,LONG D H,et al.Ultrasound assited,base-catalyzed,homogeneous reaction for ferulic acid production from γ-oryzanol[J].J Chem,2018(19):1-9.
- [13]SUN Yongyue,WANG Wenhua.Ultrasonic extraction of ferulic acid from Ligusticum chuanxiong[J].Journal of the Chinese Institute of Chemical Engineers,2008,39(6):653-656.
- [14]GRABBER J H,HATFIELD R D,RALPH J,et al.Ferulate cross-linking in cell walls isolated from maize cell suspensions[J].Phytochem,1995,40(4):1077-1082.
- [15]弓晓艳.山西老陈醋酿造过程中微生物群落结构与功能研究[D].太原:山西大学,2010.
- [16]苏静.山西老陈醋多酚类物质的提取及抗氧化活性研究[D].太原:山西大学,2010.
- [17]王元太,程光胜.山西老陈醋的工艺精华和产品特色农产品加工[J].农产品加工,2005(12):40-42.
- [18]刘凯凤,杨丽,郑胜蓝,等.山西老陈醋中优势产酸菌的分离纯化及功能分析[J].食品工业科技,2020,41(6):124-130.
- [19]SAKANAKA S,ISHIHARA Y.Comparison of antioxidant properties of persimmon vinegar and some other commercial vinegars in radical-scavenging assays and on lipid oxidation in tuna homogenates[J].Food Chemistry,2008,107(2):739-744.
- [20]VERZELLONI E,TAGLIAZUCCHI D,CONTE A.Relationship between the antioxidant properties and the phenolic and flavonoid content in traditional balsamic vinegar[J].Food Chemistry,2007,105(2):564-571.
- [21]XU Qingping,TAO Wenyi,AO Zonghua.Antioxidant activity of vinegar melanoidins[J].Food Chemistry,2007,102(3):841-849.
- [22]李丹亚,许泓瑜,陆震鸣,等.镇江香醋在陈放过程中功能因子的变化规律研究[J].食品工业科技,2008,29(7):219-221.
- [23]苗志伟,刘玉平,陈海涛,等.两种陈酿期山西老陈醋挥发性成分分析[J].食品科学,2011,31(24):380-384.
- [24]胡博涵,吴晖,赖富饶,等.产阿魏酸酯酶菌株的筛选及其酚酸释放研究[J].现代食品科技,2015,31(7):92-98.
- [25]何粉霞.小麦麸皮中阿魏酸的提取、结构修饰及生物活性的研究[D].郑州:河南工业大学,2011.