韩林,夏兵,丁博,申连长,周倩倩,徐佳雨

• 1:重庆三峡学院生命科学与工程学院
• 2:中华全国供销合作总社杭州茶叶研究院

摘要(Abstract)：

为了研究川佛手精油的抗氧化和抑菌活性,以石油醚作为溶剂超声辅助提取得到川佛手精油,测定其抗氧化活性和体外抑菌活性,川佛手精油对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、苏云金芽孢杆菌、肠球菌5种常见食源性污染菌的抑菌效果。结果表明:川佛手精油含有丰富的多酚和黄酮类物质,具有一定的抗氧化活性,对DPPH和ABTS自由基清除能力较强,EC50分别为3.92,1.88mg/mL。体外抑菌试验表明川佛手精油对肠球菌有很好的抑菌效果,最低抑菌浓度(MIC)为24mg/mL,抑菌强弱顺序为:肠球菌、苏云金芽孢杆菌、枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌。

关键词(KeyWords)： 川佛手;精油;抗氧化;抑菌

Abstract:

Keywords:

基金项目(Foundation): 重庆市教委科学技术研究项目(KJ131114);; 重庆三峡学院青年项目(12QN14)

作者(Author): 韩林,夏兵,丁博,申连长,周倩倩,徐佳雨

参考文献(References)：

• [1]赵秀玲.佛手生理活性成分的研究进展[J].食品工业科技,2012,33(21):393-399.
• [2]张福平,连少虹,陈蔚辉.佛手柑黄酮类化合物提取工艺研究[J].食品研究与开发,2005,26(6):99-101.
• [3]施长春,王建英,朱婉萍,等.佛手挥发油对哮喘的治疗作用[J].实用儿科临床杂志,2010,25(4):287-288.
• [4]麻艳芳,邵邻相,张均平,等.佛手挥发油对MDA-MB-435人乳腺癌细胞体外增殖的影响[J].中国药学杂志,2010,45(22):1737-1741.
• [5]金晓玲.佛手挥发油的研究进展[J].香料香精化妆品,2002(2):20-23.
• [6]Duan X J,Zhang W W,Li X M,et al.Evaluation of antioxidant property of extract and fractions obtained from a red alga,Polysiphonia urceolata[J].Food Chemistry,2006,95:37-43.
• [7]韩林.槟榔中抗氧化成分的提取及分离研究[D].海口:海南大学,2010.
• [8]Sun T,Ho C T.Antioxidant activities of buckwheat extracts[J].Food Chemistry,2005,90:743-749.
• [9]Fang Z X,Zhang Y H,Yuan L,et al.Phenolic compounds and antioxidant capacities of bayberry juices[J].Food Chemistry,2009,113:884-888.
• [10]Jun Liu,Cuina Wang,Zuozhao Wang,et al.The antioxidant and free-radical scavenging activities of extract and fractions from corn silk(Zea mays L.)and related flavone glycosides[J].Food Chemistry,2011,126:261-269.
• [11]Laciar A,Vaca Ruiz M L,Carrizo Flores R,et al.Antibacterial and antioxidant activities of the essential oil of Artemisia echegarayi Hieron.(Asteraceae)[J].Revista Argentina de Microbiologia,2009,41:226-231.
• [12]Rita R Kurdelas,Sandra López,Beatriz Lima,et al.Chemical composition,anti-insect and antimicrobial activity of Baccharis darwinii essential oil from Argentina Patagonia[J].Industrial Crops and Products,2012,40:261-267.
• [13]Al-Qudah M A,Al-Jaber H I,Abu Zarga M H,et al.Flavonoid and phenolic compounds from Salvia palaestina L.growing wild in Jordan and their antioxidant activities[J].Phytochemistry,2014,99:115-120.
• [14]Naciye Erkan.Antioxidant activity and phenolic compounds of fractions from Portulaca oleracea L.[J].Food Chemistry,2012,133:775-781.
• [15]Dorman H J D,Kosar M,Kahlos K,et al.Antioxidant properties and composition of aqueous extracts from Mentha species,hybrids,varieties,and cultivars[J].J.Agric.Food Chem.,2003,51:4563-4569.