植物乳杆菌5-7-3降解亚硝酸盐培养条件优化Optimization of Culture Conditions of Lactobacillus plantarum 5-7-3 Degrading Nitrite
杨晶,黄玉军,陈大卫,关成冉,姚瑶,印伯星,杨仁琴
摘要(Abstract)：
以从泡菜中筛选出的1株亚硝酸盐降解能力较高的植物乳杆菌5-7-3为研究对象,对其培养条件进行优化,提高亚硝酸盐的降解率。研究培养温度、发酵液起始pH、接种龄、接种量这些单因素对植物乳杆菌5-7-3降解亚硝酸盐效果的影响,并利用Box-Behnken中心组合试验设计原理设计植物乳杆菌5-7-3培养条件模型,得到亚硝酸盐降解率最高的培养条件:温度37℃,pH 6,接种量8%,接种龄21h。该培养条件下菌株的亚硝酸盐降解率达到89.94%,比优化前提高了4.98%。
关键词(KeyWords)： 植物乳杆菌;亚硝酸盐;降解;培养条件;优化
基金项目(Foundation): 江苏省高校自然科学研究重大项目(17KJA550004);; 国家自然科学基金青年基金项目(31700079);国家自然科学基金青年基金项目(31701627);; 江苏省高校自然科学研究面上项目(17KJB550009)
作者(Author): 杨晶,黄玉军,陈大卫,关成冉,姚瑶,印伯星,杨仁琴
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