万璐,闫佳佳,许哲祥,姜硕,郑春英

• 1:黑龙江大学农业微生物技术教育部工程研究中心
• 2:黑龙江大学生命科学学院黑龙江省普通高校微生物重点实验室

摘要(Abstract)：

以分离自黄芪根部的枯草芽孢杆菌HS8为研究对象,利用气相色谱-质谱(GC-MS)联用技术对枯草芽孢杆菌HS8的挥发性成分进行分析,在枯草芽孢杆菌HS8发酵液中分离并鉴定了21种挥发性成分;在枯草芽孢杆菌HS8菌丝体中分离并鉴定了18种挥发性成分,经对比分析,枯草芽孢杆菌HS8含有与宿主植物黄芪相同、相似及全新的挥发性成分,上述结果对开发利用枯草芽孢杆菌HS8具有重要意义。

关键词(KeyWords)： 枯草芽孢杆菌;气相色谱-质谱联用;挥发性成分;内生菌

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 万璐,闫佳佳,许哲祥,姜硕,郑春英

参考文献(References)：

• [1]Liu H W,Yin S L,An L K,et al.Complete genome sequence of Bacillus subtilis BSD-2,a microbial germicide isolated from cultivated cotton[J].Journal of Biotechnology,2016,230(5):26-27.
• [2]VavrováL,MuchováK,Barák I,et al.Comparison of different Bacillus subtilis expression systems[J].Research in Microbiology,2010,161:791-797.
• [3]Ragul K,Ibadondor Syiem,Sundar K,et al.Characterization of probiotic potential of Bacillus species isolated from a traditional brine pickle[J].J Food Sci Technol,2017,54(13):4473-4483.
• [4]Sanket J,Chirag B,Anjana J D.Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis20B[J].Bioresource Technology,2008,99:4603-4608.
• [5]Jeon H L,Lee N K,Yang S J,et al.Probiotic characterization of Bacillus subtilis P223isolated from kimchi[J].Food Sci Biotechnol,2017,26(6):1641-1648.
• [6]Rahman M S,Ano T.Production characteristics of lipopeptide antibiotics in biofilm fermentation of Bacillus subtilis[J].Journal of Environmental Sciences Supplement,2009,21(Suppl.):36-39.
• [7]Hong Ha,Huang J M,Khaneja R,et al.The safety of Bacillus subtilis and Bacillus indicus as food probiotics[J].Journal of Applied Microbiology,2008,105:510-520.
• [8]Nasim A,Marzieh H,Abolfazl P.Application of sporeforming probiotic Bacillus in the production of Nabat-a new functional sweetener[J].LWT-Food Science and Technology,2019,113:1-6.
• [9]满丽莉，向殿军．枯草芽孢杆菌MX-6的特性研究[J]．中国调味品，2019,44(9):24-28.
• [10]高娟娟，贾丽艳，田宇敏，等．枯草芽孢杆菌CGMCC6624对抗生素耐受性的研究[J]．中国调味品，2019,44(3):31-35.
• [11]满丽莉，向殿军．传统发酵豆制品中纳豆激酶产生菌的筛选及发酵培养基的优化[J]．中国调味品，2019,44(6):44-49.
• [12]吴桂苹，谷风林，朱科学，等．云南怒江草果的微波无溶剂萃取及其挥发性风味物质的GC-TOF-MS分析[J]．中国调味品，2020,45(1):172-179.
• [13]Pan H Q,Li Q L,Hu J C.The complete genome sequence of Bacillus velezensis 9912Dreveals its biocontrol mechanism as a novel commercial biological fungicide agent[J].Journal of Biotechnology,2017,247:25-28.
• [14]Lu J,Zhu X Y,Zhang C,et al.Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxifification[J].Food and Chemical Toxicology,2020,135:1-9.
• [15]魏泉增，王磊，肖付刚．GC-MS分析不同产地花椒挥发性成分[J]．中国调味品，2020,45(3):152-157.