周嘉明,姚建华,赵华锋,赵峰,李超,周德庆

• 1:青岛市华测检测技术有限公司
• 2:中国水产科学研究院黄海水产研究所

摘要(Abstract)：

建立了液相色谱-串联质谱分析深海鱼及鱼酱中太平洋雪卡毒素(P-CTX-1,P-CTX-2,P-CTX-3)的方法。用甲醇/正己烷(80/20)溶液对太平洋雪卡毒素(P-CTX-1,P-CTX-2,P-CTX-3)进行提取,C18、硅胶固相萃取柱净化后,采用液相色谱分离,以电喷雾离子源正离子选择反应监测模式进行质谱分析。三种太平洋雪卡毒素(P-CTX-1,P-CTX-2,P-CTX-3)在1.0~20μg/L线性良好,相关系数>0.99。虎鳗肌肉空白样品添加太平洋卡毒素的回收率均在70.1%~93.5%(n=6),精密度(RSD)在2.9%~15.3%。鱼肉组织中太平洋雪卡毒素(P-CTX-1,P-CTX-2,P-CTX-3)的检出限均为0.1μg/kg。

关键词(KeyWords)： 太平洋雪卡毒素;鱼肉组织;液相色谱-串联质谱

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划资助项目(22012BAK17B13)

作者(Author): 周嘉明,姚建华,赵华锋,赵峰,李超,周德庆

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