Bacillus amyloliquefaciens SY07产α-葡萄糖苷酶抑制剂液态发酵条件优化Optimization of Liquid Fermentation Conditions for the Production of α-Glucosidase Inhibitors by Bacillus amyloliquefaciens SY07
方元元,李风娟,陈勉华,李贞景,王昌禄
摘要(Abstract):
对一株解淀粉芽孢杆菌(Bacillus amyloliquefaciens SY07)产α-葡萄糖苷酶抑制剂的最适培养基和液态发酵条件进行优化。豆渣可作为该菌产α-葡萄糖苷酶抑制剂的适用培养基,优化发酵条件为豆渣5%,装液量60 mL/250 mL三角瓶,接种量2%,初始pH值6.5,摇床转速180r/min,培养温度37℃,发酵时间48 h。在此条件下,发酵产物对α-葡萄糖苷酶活性的抑制率为60.08%。B.amyloliquefaciens SY07良好地利用了豆渣产α-葡萄糖苷酶抑制剂的能力,为潜在的降血糖功能性食品的研发及农副产品的综合利用开辟了新途径。
关键词(KeyWords): 解淀粉芽孢杆菌;α-葡萄糖苷酶;抑制活性;发酵条件;豆渣;功能性食品
基金项目(Foundation): 国家自然科学基金(31101329);; 联合国大学UNU-Kirin Follow-up Research Grant(2013-15)
作者(Author): 方元元,李风娟,陈勉华,李贞景,王昌禄
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