荞麦麸皮蛋白的制备及功能特性研究Preparation and Functional Properties of Buckwheat Bran Protein
王立博,陈静,任艳娟,闫铭欢,张亚坤,王昊然,吴伟菁,罗登林
摘要(Abstract):
探究了荞麦麸皮蛋白的最佳制备工艺及功能特性差异。通过单因素实验对pH、温度、料液比和时间进行初步优化,并在此基础上采用响应面实验确定最佳工艺参数:pH为10,料液比为1∶10,时间为60 min,温度为40℃。进一步分析了温度和pH对苦荞麸皮蛋白和甜荞麸皮蛋白功能特性的影响。结果表明,随着温度升高,两种蛋白的溶解性及持水性均呈上升趋势,在60~80℃时,两种蛋白有较好的起泡性和乳化性,且稳定性良好。碱性环境(pH≥8)可提高两种蛋白质的溶解性、持水性、乳化性和乳化稳定性。与大豆分离蛋白相比,极酸(pH=2)或碱性(pH≥8)条件下,甜荞麸皮蛋白展现出良好的起泡性,而苦荞麸皮蛋白则展现出良好的泡沫稳定性。综上所述,适当热处理及碱性环境可改善荞麦麸皮蛋白的功能特性。
关键词(KeyWords): 荞麦麸皮;蛋白;单因素实验;响应面法;功能特性
基金项目(Foundation): 国家自然科学基金青年基金项目(32202096);; 河南省重点研发与推广专项(科技攻关)(232102110159);; 教育部产学合作协同育人项目(220503880205011);; 河南省重大公益专项(201300110300);; 河南科技大学博士科研启动基金(13480082)
作者(Author): 王立博,陈静,任艳娟,闫铭欢,张亚坤,王昊然,吴伟菁,罗登林
参考文献(References):
- [1]FABIIO A D,PARRAGA G.Origin,Production and Utilization of Pseudocereals[M]//Pseudocereals:Chemistry and Technology,Hoboken:Wiley Blackwell,2017:1-27.
- [2]T?M?SK?ZI S,LANGó B.Buckwheat:Its Unique Nutritional and Health-Promoting Attributes[M]//Gluten-Free Ancient Grains,Cambridge:Woodhead Publishing,2017:161-177.
- [3]QIN P,WANG Q,SHAN F,et al.Nutritional composition and flavonoids content of flour from different buckwheat cultivars[J].International Journal of Food Science & Technology,2010,45(5):951-958.
- [4]ZHU F.Buckwheat starch:structures,properties,and applications[J].Trends in Food Science & Technology,2016,49:121-135.
- [5]PULIGUNDLA P,LIM S.Buckwheat noodles:processing and quality enhancement[J].Food Science and Biotechnology,2021,30:1471-1480.
- [6]KOWALSKI S,MIKULEC A,MICKOWSKA B,et al.Nutritional properties and amino acid profile of buckwheat bread[J].Journal of Food Science and Technology,2022,59(8):3020-3030.
- [7]BRITES L T G F,ORTOLAN F,SILVA D W,et al.Gluten-free cookies elaborated with buckwheat flour,millet flour and chia seeds[J].Food Science and Technology,2018,39:458-466.
- [8]WANG J,XIAO J,LIU X,et al.Analysis of tartary buckwheat (Fagopyrum tataricum) seed proteome using offline two-dimensional liquid chromatography and tandem mass spectrometry[J].Journal of Food Biochemistry,2019,43(7):12863.
- [9]ZHOU Y,OUYANG B,DU L,et al.Effects of ultra-high-pressure treatment on the structural and functional properties of buckwheat 13S globulin[J].Food Science and Human Wellness,2022,11(4):895-903.
- [10]JANSSEN F,PAULY A,ROMBOUTS I,et al.Proteins of amaranth (Amaranthus spp.),buckwheat (Fagopyrum spp.),and quinoa (Chenopodium spp.):a food science and technology perspective[J].Comprehensive Reviews in Food Science and Food Safety,2017,16(1):39-58.
- [11]JIN J,OHANENYE I C,UDENIGWE C C.Buckwheat proteins:functionality,safety,bioactivity,and prospects as alternative plant-based proteins in the food industry[J].Critical Reviews in Food Science and Nutrition,2022,62(7):1752-1764.
- [12]WU L,LI J,WU W,et al.Effect of extraction pH on functional properties,structural properties,and in vitro gastrointestinal digestion of tartary buckwheat protein isolates[J].Journal of Cereal Science,2021,101:103314.
- [13]YU Y,LIANG G,WANG H.Interaction mechanism of flavonoids and tartary buckwheat bran protein:a fluorescence spectroscopic and 3D-QSAR study[J].Food Research International,2022,160:111669.
- [14]DU M,XIE J,GONG B,et al.Extraction,physicochemical characteristics and functional properties of mung bean protein[J].Food Hydrocolloids,2018,76:131-140.
- [15]周小理,侍荣华,周一鸣,等.热处理方式对苦荞蛋白功能特性的影响[J].农业工程学报,2018,34(9):292-298.
- [16]KAUSHIK P,DOWLING K,MCKNIGHT S,et al.Preparation,characterization and functional properties of flax seed protein isolate[J].Food Chemistry,2016,197:212-220.
- [17]BI B,YANG H,FANG Y,et al.Characterization and emulsifying properties of β-lactoglobulin-gum Acacia seyal conjugates prepared via the Maillard reaction[J].Food Chemistry,2017,214:614-621.
- [18]HAMMI K M,JDEY A,ABDELLY C,et al.Optimization of ultrasound-assisted extraction of antioxidant compounds from Tunisian Zizyphus lotus fruits using response surface methodology[J].Food Chemistry,2015,184:80-89.
- [19]DAILEY A,VUONG Q V.Optimization of aqueous extraction conditions for recovery of phenolic content and antioxidant properties from macadamia (Macadamia tetraphylla) skin waste[J].Antioxidants,2015,4(4):699-718.
- [20]XU D P,ZHENG J,ZHOU Y,et al.Ultrasound-assisted extraction of natural antioxidants from the flower of Limonium sinuatum:optimization and comparison with conventional methods[J].Food Chemistry,2017,217:552-559.
- [21]WANG W,MA X,XU Y,et al.Ultrasound-assisted heating extraction of pectin from grapefruit peel:optimization and comparison with the conventional method[J].Food Chemistry,2015,178:106-114.
- [22]李玉娥,王愈,陈振家.燕麦分离蛋白的提取及功能性测定[J].粮油食品科技,2018,26(4):31-35.
- [23]魏君慧,薛媛,冯莉,等.杏鲍菇分离蛋白和清蛋白的理化性质及功能分析[J].食品科学,2018,39(18):54-60.
- [24]ZHAO Q,HONG X,FAN L,et al.Solubility and emulsifying properties of perilla protein isolate:improvement by phosphorylation in the presence of sodium tripolyphosphate and sodium trimetaphosphate[J].Food Chemistry,2022,382:132252.
- [25]范三红,贾槐旺,张锦华,等.不同提取方法对紫苏籽粕蛋白功能性质的影响[J].中国调味品,2021,46(12):61-69.
- [26]LIU Y,ZHAO G,ZHAO M,et al.Improvement of functional properties of peanut protein isolate by conjugation with dextran through Maillard reaction[J].Food Chemistry,2012,131(3):901-906.
- [27]MA K K,GREIS M,LU J,et al.Functional performance of plant proteins[J].Foods,2022,11(4):594.
- [28]NISHINARI K,FANG Y,GUO S,et al.Soy proteins:a review on composition,aggregation and emulsification[J].Food Hydrocolloids,2014,39:301-318.
- [29]WANG S,XU X,WANG S,et al.Effects of microwave treatment on structure,functional properties and antioxidant activities of germinated tartary buckwheat protein[J].Foods,2022,11(10):1373.
- [30]HOUDE M,KHODAEI N,BENKERROUM N,et al.Barley protein concentrates:extraction,structural and functional properties[J].Food Chemistry,2018,254:367-376.