花椒与辣椒协同抗氧化作用评价研究Evaluation on the Synergistic Antioxidant Effects of Zanthoxylum bungeanum Maxim. and Capsicum annuum L.
彭青,卢云浩,何强
摘要(Abstract):
采用水提法和醇提法对花椒和辣椒的活性物质进行提取,考察了花椒和辣椒单独和联合使用对亚硝酸盐(NO_2~-·)、DPPH·和ABTS~+·的清除作用,并采用Chou-Talalay联合指数法评价其协同效果。结果显示:在NO_2~-·、DPPH·、ABTS~+·清除体系中,花椒-辣椒联合水提物和醇提物的IC_(50)值分别为308.41,224.56,54.44,33.93,346.56,200.36μg/mL,显著低于花椒和辣椒单独使用的IC_(50)值。联合指数(CI)表明,清除率从20%到95%,花椒-辣椒水提物和醇提物的CI值均小于1;等效应图说明联合使用花椒和辣椒提取物使用剂量降低;剂量减少指数对数(log DRI)分析表明,清除率从5%到95%,花椒和辣椒提取物单独使用时的log DRI均大于0,上述结果表明花椒和辣椒在同时使用时,对NO_2~-·、DPPH·和ABTS~+·等具有明显的协同清除作用。研究结果为花椒和辣椒在食品加工及餐饮中的合理使用提供了参考。
关键词(KeyWords): 花椒;辣椒;提取物;抗氧化;协同
基金项目(Foundation): 四川省重点研发项目(2019YFN0172,2020YFN0151)
作者(Author): 彭青,卢云浩,何强
参考文献(References):
- [1]BRYAN N S,ALEXANDER D D,COUGHLIN J R,et al.Ingested nitrate and nitrite and stomach cancer risk:an updated review[J].Food&Chemical Toxicology,2012,50(10):3646-3665.
- [2]MARINESCU S,ANGHEL R,GRUIA M,et al.Involvement of reactive oxygen species in the mechanisms associated with cervical cancer specific treatment[J].Chirurgia,2014,109(6):806.
- [3]GORDON M.Significance of dietary antioxidants for health[J].International Journal of Molecular Sciences,2012,13(1):173-179.
- [4]RUNDL F T,OLSSON E,WIERNIK A,et al.Potential nitrite scavengers as inhibitors of the formation of N-nitrosamines in solution and tobacco matrix systems[J].JAgric Food Chem,2000,48(9):4381-4388.
- [5]高静.天然抗氧化剂及其协同作用[J].食品安全质量检测学报,2020,11(6):1859-1864.
- [6]BADER M,STARK T D,DAWID C,et al.All-trans-coniguration in Zanthoxylumalkylamides swaps the tingling with a numbing sensation and diminishes salivation[J].J Agric Food Chem,2014,62(12):2479-2488.
- [7]NOOREEN Z,SINGH S,SINGH D K,et al.Characterization and evaluation of bioactive polyphenolic constituents fromZanthoxylum armatum DC.,a traditionally used plant[J].Biomedicine&Pharmacotherapy,2017,89:366.
- [8]TU X F,HU F,THAKUR K,et al.Comparison of antibacterial effects and fumigant toxicity of essential oils extracted from different plants[J].Industrial Crops and Products,2018,124:192-200.
- [9]BAENAS N,BELOVIC M,ILIC N,et al.Industrial use of pepper (Capsicum annum L.)derived products:technological benefits and biological advantages[J].Food Chemistry,2019,274:872-885.
- [10]LU Y,DONG Y,LI X,et al.The nitrite-scavenging properties of catechol,resorcinol,and hydroquinone:a comparative study on their nitration and nitrosation reactions[J].Journal of Food Science 2016,81(10-12):2692-2696.
- [11]曾维才,石碧.天然产物抗氧化活性的常见评价方法[J].化工进展,2013(6):1205-1213.
- [12]CHOU T C.Drug combination studies and their synergy quantification using the Chou-Talalay method[J].Cancer Research,2010,70(2):440.
- [13]FOTI M C.The use and abuse of the DPPH·radical[J].Journal of Agricultural&Food Chemistry,2015,63(40):8765.
- [14]MAKSIMOVA V,MIRCESKI V,GULABOSKI R,et al.Electrochemical evaluation of the synergistic effect of the antioxidant activity of capsaicin and other bioactive compounds in Capsicumsp.extracts[J].International Journal of Electrochemical Science,2016,11(8):6673-6687.
- [15]HAJIMEHDIPOOR H,SHAHRESTANI R,SHEKARCHIM.Investigating the synergistic antioxidant effects of some flavonoid and phenolic compounds[J].Research Journal of Pharmacognosy,2014,1(3):35-40.