双菌株在酱油制曲中的应用Application of Two Strains in Koji-making of Soy Sauce Fermentation
张齐军
摘要(Abstract):
研究以豆饼、麸皮为原料,利用米曲霉、黑曲霉双菌株进行酱油制曲,通过单因素实验和正交试验确定了酱油制曲工艺中最合适的培养条件。结果表明:选用米曲霉、黑曲霉种曲配比为6∶2,将双菌株以2%的接种量接入到曲料中,控制制曲温度32℃,制曲时间43h,得到的成曲蛋白酶酶活力高达2787U/g,得到的成曲菌丝丰满,曲味芳香。
关键词(KeyWords): 米曲霉;黑曲霉;蛋白酶酶活;制曲工艺
基金项目(Foundation):
作者(Author): 张齐军
参考文献(References):
- [1]邹镜铭.多菌种混合制曲提高酱油质量[J].中国调味品,2005(4):33-34.
- [2]Zhang Y Y,Tao W Y.Flavor and taste compounds analysis in Chinese solid fermented soy sauce[J].African Journal of Biotechnology,2009,8(4):673-681.
- [3]毋瑾超,胡锡钢,陈全震.酱油发酵中制曲条件对制曲效果的影响[J].粮油食品科技,2004,12(2):7-8.
- [4]Tatautro U,Yoshiharu N,Yuji T.Practical soy sauce production using a mixed koji-making system[J].J Ferment Bioeng.1994,78(3):262-264.
- [5]Kljima K,Suzuki H.Improving the umami taste of soy sauce by the addition of bacterialγ-glutamyltranspeptidase as a glutaminade to the fermentation mixture[J].Enzyme Microb Tech,2007,41:80-84.
- [6]Susana R C,Mangeles S.Application of solid-state fermentation to food industry[J].Journal of Food Engineering,2006(76):291-302.
- [7]王素珍.利用双菌种制曲提高原料蛋白质利用率的探讨[J].中国酿造,2005(7):35-37.
- [8]张艳芳,陶文沂.米曲霉双菌株组合制曲改善酶系组成与发酵效果研究[J].食品与发酵工业,2008,34(9):37-39.
- [9]Su N W,Wang M L,Kwok K F.Effects of temperature and sodium chloride concentration on the activities of proteases and amylases in soy sauce koji[J].Journal of Agriculture and Food Chemistry,2005,53(5):1521-1525.
- [10]Anson M L.The estimmate of pepsin,trypsin,papain,and cathepsin with hemoglobin[J].The Journal of General Physiology,1938,22(1):79.