百香果皮总黄酮提取工艺优化及其抗氧化活性研究Optimization of Extraction Technology of Total Flavonoids from Passiflora edulis Sims Peel and Study on the Antioxidant Activity
景继月,许梦圆,田永涛,田海龙,王文蜀
摘要(Abstract):
采用超声辅助溶剂法提取百香果皮中的总黄酮。基于单因素实验结果,以浸膏得率、总黄酮含量和清除DPPH自由基能力多指标为响应值,应用响应面法综合优化总黄酮提取工艺。结果表明,总黄酮最佳工艺为液料比40∶1,乙醇百分含量67%,超声时间30 min。此条件下浸膏得率为12.58%,总黄酮含量为11.56 mg RT/g DW,清除DPPH自由基SC_(50)值为0.19 mg/mL。最优提取工艺下的百香果皮黄酮提取物具有较好的清除ABTS自由基((0.17±0.01) mmol/L TE/g)和铁离子还原能力((2.35±0.03) mmol/L Fe~(2+)/g)。
关键词(KeyWords): 百香果皮;总黄酮提取;响应面优化;抗氧化活性
基金项目(Foundation): 国家民委创新项目团队(10301-202000040203);; 建设世界一流大学(学科)和特色发展引导专项(YLDXXK201819);; 中央民族大学生命与环境科学学院生物成像与系统生物学研究中心(104-01900403);中央民族大学创新项目(URTP2021110046,URTP2021110082,URTP2021110062)
作者(Author): 景继月,许梦圆,田永涛,田海龙,王文蜀
参考文献(References):
- [1]DA SILVA LIMA L K,DE JESUS O N,SOARES T L,et al.Growth,physiological,anatomical and nutritional responses of two phenotypically distinct passion fruit species (Passiflora L.) and their hybrid under saline conditions[J].Scientia Horticulturae,2019,263:109037-109052.
- [2]朱洁,龙秀琴,蔡国俊,等.两个品种百香果营养成分比较分析[J].湖北农业科学,2017,56(13):2476-2478.
- [3]LUCENA G I,DE Sá BARRETO D C M,VERAS OLIVEIRA D C M,et al.A systematic review on phenolic compounds in Passiflora plants:exploring biodiversity for food,nutrition,and popular medicine[J].Critical Reviews in Food Science and Nutrition,2018,58(5):785-807.
- [4]梁倩,李咏富,龙明秀,等.百香果化学成分及药理活性研究进展[J].食品工业科技,2018,39(20):343-347.
- [5]董龙,王小媚,蔡昭艳,等.广西百香果产业现状及存在的问题与发展建议[J].南方园艺,2020,31(6):76-78.
- [6]HU Y,JIAO L,JIANG M H,et al.A new C-glycosyl flavone and a new neolignan glycoside from Passiflora edulis Sims peel[J].Natural Product Research,2018,32(19/21):2312-2318.
- [7]SILVA R O,DAMASCENO S R B,BRITO T V,et al.Polysaccharide fraction isolated from Passiflora edulis inhibits the inflammatory response and the oxidative stress in mice[J].Journal of Pharmacy and Pharmacology,2015,67(7):1017-1027.
- [8]AYRES A,SANTOS W B,JUNQUEIRA-AYRES D D,et al.Monoaminergic neurotransmission is mediating the antidepressant-like effects of Passiflora edulis Sims fo.edulis[J].Neuroscience Letters,2017,660:79-85.
- [9]LIMA G C,VUOLO M M,BATISTA A G,et al.Passiflora edulis peel intake improves insulin sensitivity,increasing incretins and hypothalamic satietogenic neuropeptide in rats on a high-fat diet[J].Nutrition,2016,32(7/8):863-870.
- [10]KLEIN N,GAZOLA A C,LIMA T C M D,et al.Assessment of sedative effects of Passiflora edulis f.flavicarpa and Passiflora alata extracts in mice,measured by telemetry[J].Phytotherapy Research,2014,28(5):706-713.
- [11]CAZARIN C B,SILVA J K D,COLOMEU T C,et al.Passiflora edulis peel intake and ulcerative colitis:approaches for prevention and treatment[J].Experimental Biology and Medicine,2014,239(5):542-551.
- [12]李欣燃,张华兴,翁贵英,等.百香果黄酮提取工艺优化及其抗氧化活性研究[J].食品工业科技,2020,41(24):106-112.
- [13]陶永元,舒康云,董洪丽,等.西番莲黄酮的提取及抗氧化活性研究[J].食品研究与开发,2014,35(17):25-29.
- [14]廖兰,吴俊良.逆流法提取百香果果皮中总黄酮的工艺研究[J].广西科技大学学报,2018,29(2):91-96.
- [15]王珊.新型提取技术在天然活性成分分析中的应用[D].杭州:浙江工业大学,2019.
- [16]KIVRAK ?.Essential oil composition and antioxidant activities of eight cultivars of Lavender and Lavandin from western Anatolia[J].Industrial Cropsand Products,2018,117:88-96.
- [17]LI X C,LIN J,GAO Y X,et al.Antioxidant activity and mechanism of Rhizoma Cimicifugae[J].Chemistry Central Journal,2012,6(1):140.
- [18]BENZIE I F,STRAIN J J.The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”:the FRAP assay[J].Analytical Biochemistry,1996,239(1):70-76.
- [19]石峰,李思敏,高定烽,等.基于真空冷冻干燥的响应面优化超声提取青杨梅中抗氧化物质及活性分析[J].中国调味品,2021,46(4):43-49.
- [20]郑清,陈宝印,杨磊,等.响应面法优化同步提取黑蒜中多酚与黄酮的工艺及抗氧化活性的测定[J].中国调味品,2021,46(5):69-76.
- [21]陈建福,彭敏.芦柑叶总黄酮的提取工艺优化及其抗氧化活性研究[J].食品研究与开发,2021,42(4):117-122.
- [22]杨申明,王振吉,杨红卫.香椿树皮总黄酮提取工艺优化及抗氧化特性[J].粮食与油脂,2021,34(2):128-132.
- [23]赵玉红,师帅帅,张立钢.‘鲁赫’刺蔷薇叶不同溶剂提取物的活性成分及抗氧化性比较[J].现代食品科技,2019,35(5):159-166.
- [24]余启明,陈昌盛,廖娟,等.百香果果皮的微波预处理-超声波提取工艺及其抗氧化活性研究[J].化工技术与开发,2019,48(11):18-21.