辛香料植物贯叶马兜铃的根及叶挥发性成分分析及其食用风险解析Research on the Volatiles from Leaves and Roots of Aristolochia delavayi Franch.and Their Potential Safety Hazards
陈高,葛佳,秦燕,孙卫邦
摘要(Abstract):
采用气相色谱-质谱联用技术对动态吸附法收集的贯叶马兜铃(Aristolochia delavayi Franch.)干燥叶和根的挥发性成分进行了分析,并用气相色谱面积归一化法对各成分进行了定量。从贯叶马兜铃叶中分离出19个挥发性成分,占挥发性成分总量的97.8%,其中反式-2-葵烯醛(79.79%)、3-十二烯醛(4.26%)、辛醛(2.49%)、反式-2-己烯醛(2.09%)和葵醛(1.80%)为主要成分。从该植物根中分离出16个挥发性成分,占挥发性成分总量的98.2%。其中乙酸龙脑酯(37.08%)、尼泊金丙酯(22.29%)、1,3,8-p-孟三烯(19.93%)、莰烯(6.39%)和β-蒎烯(2.21%)为主要成分。该研究结合当地居民的食用习惯,具体阐明了自然条件下贯叶马兜铃的叶及根的辛香味成分及组成,同时结合该植物不同部分的马兜铃酸的含量,讨论了食用该植物可能存在的健康安全风险。该研究结果能为今后科学食用及保护该濒危物种提供技术指导和理论支持。
关键词(KeyWords): 马兜铃酸;贯叶马兜铃;达摩麝凤蝶;动态吸附;气相色谱-质谱
基金项目(Foundation): 国家自然科学基金NSFC-云南省联合基金(U1602264);; 云南省中青年学术和技术带头人后备人才计划(2015HB091);; 科技部专项(2017FY100100)
作者(Author): 陈高,葛佳,秦燕,孙卫邦
参考文献(References):
- [1]张卫明,肖正春.中国辛香料植物资源开发与利用[M].南京:东南大学出版社,2007:117-118.
- [2]郭振锋,董利萍,蔡国军,等.我国的主要辛香料资源及开发利用[J].中国林副特产,2009,22(2):68-72.
- [3]张卫明,史劲松,顾龚平.我国辛香料植物资源及其利用[J].云南植物研究,2004,15(1):70-79.
- [4]腾跃.天然香料中的瘟魔[J].食品与健康,2004,13(5):20.
- [5]Debelle F D,Vanherweghem J L,Nortier J L.Aristolochic acid nephropathy:a worldwide problem[J].Kidney International,2008,74(2):158-169.
- [6]Yang Z Y,Yi T S,Zeng L Q,et al.The population genetic structure and diversification of Aristolochia delavayi(Aristolochiaceae),an endangered species of the dry hot valleys of the Jinsha River,southwestern China[J].Botany,2014,92(8):579-587.
- [7]Chen G,Luo S,Mei N,et al.Case study of building of conservation coalitions to conserve ecological interactions[J].Conservation Biology,2015,29(6):1527-1536.
- [8]周铁生,杨庆宽,张正居,等.云南山草果精油化学成分及香气的研究[J].香料香精化妆品,1995,22(3):13-17.
- [9]Vanherweghem J L,Depierreux M,Tielemans C,et al.Rapidly progressive interstitial renal fibrosis in young women:association with slimming regimen including Chinese herbs[J].Lancet,1993,341(8842):387-391.
- [10]Balachandran P,Wei F,Lin R C,et al.Structure activity relationships of aristolochic acid analogues:toxicity in cultured renal epithelial cells[J].Kidney International,2005,67(5):1797-1805.
- [11]Li Z J,Njateng G S S,He W J,et al.Chemical composition and antimicrobial activity of the essential oil from the edible aromatic plant Aristolochia delavayi[J].Chemistry&biodiversity,2013,10(11):2032-2041.
- [12]陈高,孙卫邦.动态吸附与气相色谱-质谱(GC-MS)联用分析密蒙花(醉鱼草科)的挥发性花香成分[J].植物分类与资源学报,2011,33(2):235-238.
- [13]和文佳,李志坚,张红霞,等.山草果无菌苗和愈伤组织挥发性成分研究[J].香料香精化妆品,2017,44(1):5-8.
- [14]Matsubara E,Fukagawa M,Okamoto T,et al.(-)-Bornyl acetate induces autonomic relaxation and reduces arousal level after visual display terminal work without any influences of task performance in low-dose condition[J].Biomedical Research,2011,32(2):151-157.
- [15]陈国安,杨凯,彭昌亚,等.新型食品防腐剂——尼泊金酯[J].中国调味品,2003(3):31-36.
- [16]Gold L S,Slone T H.Aristolochic acid,an herbal carcinogen,sold on the web after FDA alert[J].New England Journal of Medicine,2003,349(16):1576-1577.
- [17]Grollman A P.Aristolochic acid nephropathy:harbinger of aglobal iatrogenic disease[J].Environmental and Molecular Mutagenesis,2013,54(1):1-7.
- [18]Francisco C S,Messiano G B,Lopes L M X,et al.Classification of Aristolochiaspecies based on GC-MS and chemometric analyses of essential oils[J].Phytochemistry,2008,69(1):168-175.
- [19]Opitz S E W,Müller C.Plant chemistry and insect sequestration[J].Chemoecology,2009,19(3):117-154.