张倩倩,管于平,刁爱坡,孙媛霞

• 1:天津科技大学生物工程学院
• 2:中国科学院天津工业生物技术研究所

摘要(Abstract)：

利用摇瓶发酵优化产朊假丝酵母的培养条件,并放大至5L发酵罐进一步优化,旨在获得更高的菌体量。摇瓶发酵试验结果表明:产朊假丝酵母的最适接种量为5%,(NH4)2SO4和KH2PO4的最佳浓度分别为5g/L和10g/L,发酵温度为28℃。此外,通过对5L发酵罐的进一步优化发现:当初始葡萄糖浓度为30g/L,发酵时间为10h时,以100mL/h的速度连续流加浓度为90g/L的葡萄糖母液10h,产朊假丝酵母的生物量及收率达到最大值,分别为24.01g/L和0.49g/g,明显高于分批发酵模式的16.39g/L和0.44g/g。

关键词(KeyWords)： 产朊假丝酵母;优化;补料分批发酵;生物量;收率

Abstract:

Keywords:

基金项目(Foundation): 国家高技术发展计划(2012AA021403);; 滨海新区科技项目(2011-BK120045);; 天津市科技计划项目(11ZCKFNC02000)

作者(Author): 张倩倩,管于平,刁爱坡,孙媛霞

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