产氨基甲酸乙酯降解酶酵母菌的筛选鉴定及发酵条件优化Screening and Identification of Yeasts Producing EC-Degrading Enzymes and Optimization of Fermentation Conditions
周雪燕,辛敏汉,肖婧,程卫东,史学伟
摘要(Abstract)：
采用选择性培养基,从36株酵母菌中分离纯化得到4株(Y4、Y9、Y13、N6)产氨基甲酸乙酯降解酶酵母菌,通过ITS序列比对,均属于Pichia kudriavzevii。通过单因素试验和正交试验,对Y13菌株产EC水解酶的发酵条件进行优化。结果表明:最优条件是以10g/L麦芽糖为碳源;以30g/L酵母浸粉为氮源;接种量为6%;培养基中EC的底物浓度为5.00%,装液量为20%,初始pH为6,培养温度为28℃,培养时间为120h。
关键词(KeyWords)： 氨基甲酸乙酯;酵母菌;发酵条件优化
基金项目(Foundation): 兵团科技攻关计划(2016AB009);兵团科技攻关计划(2016AD024);; 青年科学基金项目(31500092);青年科学基金项目(31560446);; 高层次人才科研启动资金专项(RCZX201526)
作者(Author): 周雪燕,辛敏汉,肖婧,程卫东,史学伟
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