永川毛霉型豆豉中脂肪酸提取方法比较研究Comparative Study on Extraction Methods of Fatty Acids in Yongchuan Mucor-fermented Douchi
雷雨和,眭玥,骞宇,李希宇,陈炼红,黄仕友,索化夷
摘要(Abstract)：
永川毛霉型豆豉中脂肪酸的构成以及不饱和脂肪酸的含量高低影响着其保健功能的高低。研究以永川毛霉型豆豉发酵过程中4个不同阶段的原料为样本,采用了简便和国标两种方法提取脂肪酸,GC-MS分析后对两组数据进行成对数据T检验。结果显示两组数据无显著性差异,简便方法可以替代国标的前处理方法。
关键词(KeyWords)： 永川豆豉;游离脂肪酸提取;方法
基金项目(Foundation): 国家自然科学基金青年科学基金项目(31201411);; 西南大学博士基金项目(SWU112057)
作者(Author): 雷雨和,眭玥,骞宇,李希宇,陈炼红,黄仕友,索化夷
参考文献(References)：
- [1]Takahashi R,Ohmori R,Kiyose C,et al.Antioxidant activities of black and yellow soybean against low density lipoprotein oxidation[J].Journal of Agricultural and Food Chemistry,2005,53(11):4578-4582.
- [2]Astadi I R,Astuti M,Santoso U,et al.In vitro antioxidant activity of anthocyanins of black soybean seed coat in human low density lipoprotein(LDL)[J].Food Chemistry,2009,112(3):659-663.
- [3]Azevedo L,Gomes J C,Stringheta P C,et al.Black bean(Phaseolus vulgaris L.)as a protective agent against DNA damage in mice[J].Food and Chemical Toxicology,2003,41(12):1671-1676.
- [4]Hung Y H,Huang H Y,Chou C C.Mutagenic and antimutagenic effects of methanol extracts of unfermented and fermented black soybeans[J].International Journal of Food Microbiology,2007,118(1):62-68.
- [5]Gill I,Valivety R.Polyunsaturated fatty acids,Part 1:Occurrence,biological activities and applications[J].Trends Biotechnology,1997,15(10):401-409.
- [6]吴时敏.功能性油脂[M].北京:中国轻工业出版社,2001.
- [7]郑建仙.功能性食品[M].北京:中国轻工业出版社,1997.
- [8]Horrobin D F.Omega-6essential fatty acids:Pathophysiology and roles in clinical medicine[M].New York:Alan R.Liss,Inc.,1990.
- [9]De la Torre A,Debiton E,Durand D,et al.Conjugated linoleic acid isomers and their conjugated derivatives inhibit growth of human cancer cell lines[J].Anticancer Res.,2005,26(6B):3943-3949.
- [10]Sauer L A,Dauchy R T,Blask D E,et al.Eicosapentaenoic acid suppresses cell proliferation in MCF-7 human breast cancer xenografts in nude rats via a pertussis toxinsensitive signal transduction pathway[J].J Nutr.,2005,135(9):2124-2129.
- [11]Allred C D,Talbert D R,Southard R C,et al.PPAR gammal as amolecular target of eicosapentaenoic acid in human colon cancer(HT-29)cell[J].J Nutr.,2008,138(2):250-256.
- [12]GB/T 5009.6-2003,食品中脂肪的测定[S].
- [13]GB/T 17376-2008,动植物油脂脂肪酸甲酯制备[S].
- [14]刘兵,王程,金剑,等.生殖生长期遮阴对大豆脂肪酸积累动态的影响[J].大豆科学,2009,28(1):53-58.
- [15]马淑英,梁歧,尹田夫,等.大豆籽粒发育过程中脂肪酸的组分分析[J].大豆科学,1999,18(2):31-36.
- [16]刘丽君,孙聪姝,董守坤,等.硫对大豆籽粒蛋白质和脂肪组分的影响[J].大豆科学,2008,27(6):993-996.
- [17]Stredansky M,Stredanska S,Zanetti F,et al.GammaLinolenic acid production with Thamnidium elegans by solidstate fermentation on apple pomace[J].Bioresource Technology,2000,73(1):41-45.
- [18]Elena Conti,Miroslav Stredansky,Silvia Stredanska,et al.γ-Linolenic acid production by solid-state fermentation of Mucorales[J].Bioresource Technology,2001,76(3):283-286.
- [19]王卫卫,任鹏康.少根根霉C-亚麻酸高产菌株选育及发酵条件优化[J].菌物系统,2002,21(1):92-97.